We narrowed to 10,667 results for: plasmids 101
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Plasmid#230152PurposeAiE1014h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2-P2A-3XFLAG-10aa-H2B
UseAAVPromoterminBGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP13473 - pAAV-AiE1017h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3473)
Plasmid#230153PurposeAiE1017h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2-P2A-3XFLAG-10aa-H2B
UseAAVPromoterminBGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV sgRNA Expression Plasmid
Plasmid#174540PurposeContains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.DepositorTypeEmpty backboneUseAAVTagsNoneExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Noodles Plasmid
Plasmid#212613PurposeAn all-in-one system for on-target efficiency analysis of CRISPR guide RNAsDepositorTypeEmpty backboneUseCRISPR and LuciferaseExpressionMammalianAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsExpressionBacterialPromoterPgyrAAvailable SinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IL2sp-6His-Asprosin plasmid
Plasmid#190683PurposeExpresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.DepositorInsertIL2-6xHis-Asprosin
TagsIL2 signal peptide, 6xHisExpressionMammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
eIF3k sgRNA-2 PX459 plasmid
Plasmid#192256Purposeexpressing sgRNA-2 targeting eIF3k locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k sgRNA-1 PX459 plasmid
Plasmid#192255Purposeexpressing sgRNA-1 targeting eIF3k locus closed to stop codenDepositorInsertsgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK289 (mAID-mClover-NeoR) plasmid
Plasmid#192243Purposeknockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)
ExpressionMammalianAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192244Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)
ExpressionMammalianAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAX6 donor plasmid
Plasmid#105239PurposeKnock in H2B-GFP into the human PAX6 locusDepositorInsertPAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
UseTALENAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tn7 helper plasmid
Plasmid#141161PurposeTemperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoterDepositorInsertTn7 transposon machinery
ExpressionBacterialPromoterArabinose-inducible pBADAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRD4 CRISPRi plasmid
Plasmid#154890PurposeHuman BRD4 CRISPRi gRNADepositorInsertBRD4-targeting gRNA
UseCRISPR and LentiviralAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CJ8421_04975 Rptr2 recording plasmid
Plasmid#194211PurposeCJ8421_04975 Rptr2 targeted DNA sequenceDepositorInsertCJ8421_04975 Rptr2 DNA target
ExpressionBacterialAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Non-targeting CRISPRi plasmid
Plasmid#154891PurposeHuman non-targeting CRISPRi gRNADepositorInsertNontargeting gRNA
UseCRISPR and LentiviralAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAX6 CKO 2/7 Donor plasmid
Plasmid#68467Purposedonor plasmid for targeting PAX6 locusDepositorInsertFRT flanked exon4 of PAX6 gene (PAX6 Human)
UseCre/LoxExpressionMammalianPromotermouse PGK promoterAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedAvailable SinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-hL1-5'_3.3kb plasmid
Plasmid#226006PurposePlasmid used to generate DNA-FISH probe targeting at human L1HSDepositorInsertHuman L1 element 3.3kb sequences at the 5' end
UseSynthetic BiologyAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
eIF3f sgRNA-1 PX459 plasmid
Plasmid#192253Purposeexpressing sgRNA-1 targeting eIF3f locus closed to stop codenDepositorInsertsgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3f sgRNA-2 PX459 plasmid
Plasmid#192254Purposeexpressing sgRNA-2 targeting eIF3f locus closed to stop codenDepositorInsertsgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
UseCRISPRExpressionMammalianAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only