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Showing: 361 - 380 of 9391 results

  2. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  4. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    68709-plasmid-map-sequence-id-125636

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  5. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110916-plasmid-map-sequence-id-212580

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  6. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Use
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  7. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Use
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  8. pUB39

    Plasmid
    #26300
    Depositor
    George Cross
    Insert
    VSG117 (Lister 427-4) cDNA
    Use
    Trypanosome inducible expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 16, 2010
    Availability
    Academic Institutions and Nonprofits only
    26300-plasmid-map-sequence-id-12384

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  9. pME-QF2

    Plasmid
    #83307
    Purpose
    codes for QF2 (truncated QF) in a gateway middle entry backbone.
    Depositor
    Marnie Halpern
    Insert
    QF
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    83307-plasmid-map-sequence-id-173284

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  10. pAIP4_2H7W

    Plasmid
    #234220
    Purpose
    Heterologous protein expression of chagasin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234220-plasmid-map-sequence-id-464185

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  11. PP-ASL

    Plasmid
    #40749
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Trypanosoma Brucei Adenylosuccinate Lyase
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pJK383

    Plasmid
    #72474
    Purpose
    Produces Thermoplasma acidophilum citrate synthase with Asp317>Gly mutant (TpCS-D317G)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-17 to phenylalanine, tryptopha…
    Promoter
    recA
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72474-plasmid-map-sequence-id-140430

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  17. pTcINDEX-GW

    Plasmid
    #234713
    Purpose
    Used to express any CDS in T. cruzi in a tetracycline regulatable manner. Modified from Taylor et al. (2006) for easy cloning using Gateway recombination cassette.
    Depositor
    Esteban Serra
    Article
    Alonso et al Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1
    Type
    Empty backbone
    Use
    Trypanosomatid expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    234713-plasmid-map-sequence-id-473972

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Showing: 361 - 380 of 9391 results