We narrowed to 13,574 results for: cas9 genes

1. pBHM2403

   Plasmid

   #171687

   Purpose

   pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

   Depositor

   Hiten Madhani

   Article

   Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

   Insert

   CnoCAS9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TEF1

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKEE401

   Plasmid

   #91715

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXEE401B

   Plasmid

   #91716

   Purpose

   CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM51323

   Plasmid

   #153224

   Purpose

   Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV/Guide-mCherry

   Plasmid

   #170543

   Purpose

   Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV.5’eGFP/sgLMNA

   Plasmid

   #170546

   Purpose

   Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   sgRNA targeting 5'- end of LMNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Cetn1/1

   Plasmid

   #50718

   Purpose

   pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Inserts

   Cetn1 sgRNA1
   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and hU6

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. c3GIC9

   Plasmid

   #62191

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAW-AlphaLobe

    Plasmid

    #113255

    Purpose

    E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

    Depositor

    Jennifer Doudna

    Article

    Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    S. pyogenes Cas9 alpha-helical lobe
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    residues 56–714; See paper for description

    Promoter

    T7

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLdCH

    Plasmid

    #84291

    Purpose

    Express gRNA and Cas9 in Leishmania with Hygromycin resistance

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    L. donovani ribosome RNA promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCasKP-hph

    Plasmid

    #117232

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasKP-apr

    Plasmid

    #117231

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-CMV-mCherry-T2A-GFP

    Plasmid

    #109427

    Purpose

    A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99679

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTL-Bag2T-gRNA2

    Plasmid

    #196253

    Purpose

    Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    CRISPR-Cas9 guide RNA scaffold and multiple cloning site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179059

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179072

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)-Link-2xPH(PLCd)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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