We narrowed to 21,977 results for: Sis
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Plasmid#82428PurposePlasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED 11W11A
UseTagsGSTExpressionMammalianMutationamino acids 1-1158 of nvGW182 were deleted and Tr…PromoterEF1AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1526
Plasmid#82429PurposePlasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED 11W11A
UseTagsNHAExpressionMammalianMutationamino acids 1-1158 of nvGW182 were deleted and Tr…PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK5 CBM-E
Plasmid#53169PurposeExpresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
UseTagsHalo and His6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL_GI_PCB28
Plasmid#98745PurposeFor phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.DepositorInsertsUseSynthetic BiologyTagsExpressionYeastMutationCodon optimized for Saccharomyces cerevisiaePromoterADH1m and PGK1Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI-D10R-E48R
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-PGBD5
Plasmid#78121PurposeLentiviral gene expression vector for doxycycline inducible PGBD5 expressionDepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-Sec61B
Plasmid#104177PurposeExpresses fusion of CIB1 (1-170) with GFP and Sec61BDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRTagsExpressionMammalianMutationPromoterPgkAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRECLV103_PGBD5_B
Plasmid#168053PurposeLentiviral expression vector for fusion of GFP with human PGBD5, NCBI: NM_001258311.1DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-neo-YAP
Plasmid#166468PurposePlasmid coding for YAP and neomycin resistance in a lentiviral backbone.DepositorInsertYAP, Neo (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF
Plasmid#100280PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
UseTagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Myc-PGK-Puro-IRES-GFP
Plasmid#75124PurposeRetroviral expression plasmid encoding wild type murine MycDepositorInsertmyelocytomatosis oncogene (Myc Mouse)
UseRetroviralTagsNoneExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA NC
Plasmid#176259PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI andDepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero20
Plasmid#187930PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero20DepositorInsertHero20 (C11orf58 Human)
UseTagsFlag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-N289A-H290A
Plasmid#82032PurposePlasmid for constitutive or doxy-inducible expression of N289A+H290A mutant of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
UseTagsHAExpressionMammalianMutationEndonuclease-dead mutant: N289A+H290A CtIP; Mutat…PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only