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piRFP670-N1

Plasmid

#45457

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

iRFP670

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pRL623

Plasmid

#58494

Purpose

helper plasmid for bacterial conjugal DNA transfer

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Insert

encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120

Available Since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only

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YFP-CaaX

Plasmid

#155233

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

YFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control

Tags

YFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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mito-gTEMP_pcDNA3

Plasmid

#109117

Purpose

Mitochondria targetting gTEMP

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

mito-gTEMP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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RCASBP-AP (A) (CC#37)

Plasmid

#15160

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Rac1

Plasmid

#180581

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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RINS1

Plasmid

#107290

Purpose

Plasmid for the expression of the RINS1 sensor

Depositor

Dmytro Yushchenko

Article

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRPF185

Plasmid

#106367

Purpose

C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

Depositor

Robert Fagan, Neil Fairweather

Article

Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

Insert

Term(fdx)-Ptet-gusA-Term(slpA) (gusA )

Use

E. coli - c. difficile shuttle vector

Mutation

codon optimised for C. difficile

Promoter

Ptet

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-3xFLAG-empty-pgk-hph

Plasmid

#48754

Purpose

PiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pDisplay-SNAPtag

Plasmid

#205094

Purpose

Display SNAPtag on the surface of mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

SNAPtag

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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iRFP-PAmCherry1-PH_GRP1

Plasmid

#92168

Purpose

PI(3,4,5)P3 single molecule fluorescent reporter

Depositor

Xuelin Lou

Article

Ji et al J Biol Chem. 2015 Nov 6;290(45):26978-93

Insert

iRFP-PAmCherry1-PHgrp1

Tags

iRFP-PAmCherry1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP713

Plasmid

#45468

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF331_1

Plasmid

#135739

Purpose

Encodes gRNA for 3' target of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 gRNA (ZNF331 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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