237,468 results

1. pmr101A-cPr-mCherry (KmR)

   Plasmid

   #177207

   Purpose

   Plasmid with p15A origin for constitutive mCherry expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   Synthetic constitutive promoter

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCSG17-NleL

   Plasmid

   #66716

   Purpose

   Expresses NleL (170-782) in E. coli

   Depositor

   David Komander

   Article

   Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

   Insert

   NleL (NEWENTRY )
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMCSG17-NleL

   Plasmid

   #66716

   Purpose

   Expresses NleL (170-782) in E. coli

   Depositor

   David Komander

   Article

   Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

   Insert

   NleL (NEWENTRY )
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

4. LentiAsCpf1-3xMYC-blast

   Plasmid

   #127750

   Purpose

   Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Insert

   AsCpf1
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF1a-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223252

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBTK1017 (pNR)

   Plasmid

   #183131

   Purpose

   Control plasmid that does not express dsRNA

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al Science. 2020 Jan 31;367(6477):573-576.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pETM33_Nsp10

   Plasmid

   #156479

   Purpose

   Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

   Plasmid

   #84250

   Purpose

   Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

   Plasmid

   #84250

   Purpose

   Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Insert

   Sa sgTRE3G
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOPINS-AREL1

    Plasmid

    #66710

    Purpose

    Expresses AREL1 HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KIAA0317 (AREL1 )
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only