We narrowed to 11,622 results for: VARS

1. pPKm-102

   Plasmid

   #90493

   Purpose

   pcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mOrange
   

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pV1464

   Plasmid

   #111440

   Purpose

   N. castellii Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBad-CA TAG20

   Plasmid

   #105666

   Purpose

   expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tag

   Depositor

   Ryan Mehl

   Article

   Mehl et al 2020

   Insert

   human carbonic anhydrase II thermostable variant (CA2 Human)
   

   Tags

   6x his tag

   Expression

   Bacterial

   Mutation

   TAG stop codon at site 20

   Promoter

   pBad/arabinose

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GTH-ir

   Plasmid

   #81107

   Purpose

   Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Insert

   tdiRFP
   

   Use

   Cre/Lox

   Tags

   tdiRFP

   Expression

   Yeast

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV Aquamarine-superYFP tandem

   Plasmid

   #157776

   Purpose

   Produces a fusion between Aquamarine and superYFP. It can be used as a positive control for FRET (using FLIM)

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Type

   Empty backbone

   Tags

   Aquamarine and superYFP

   Expression

   Mammalian

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-2HMG-eGFP

   Plasmid

   #112737

   Purpose

   Express 2HMG-eGFP in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al RSC Adv, 2016, 6, 46291-46298

   Insert

   2HMG-eGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221 Cas9-RFC5

   Plasmid

   #183200

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-RFC5
   

   Use

   CRISPR; Gateway entry clone

   Mutation

   n/a

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti CRISPR sgSHMT2_1

   Plasmid

   #106308

   Purpose

   Express Cas9 and sgRNA targeting SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pL0_EF-H4

   Plasmid

   #202178

   Purpose

   Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   H4 terminator
   

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 28, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pL0_EF-fcpA

    Plasmid

    #202164

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpA terminator
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pL0_AC-H4

    Plasmid

    #202122

    Purpose

    Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 promoter
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pL0_AC-P49

    Plasmid

    #202120

    Purpose

    Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    P49 promoter
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159869

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p35S::mCh-HA-P2A-lifeact-mCh

    Plasmid

    #135234

    Purpose

    constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    mCh-HA-P2A-lifeact-mCh
    

    Tags

    HA, lifeact, and p2a

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-2HMG-mCherry

    Plasmid

    #112739

    Purpose

    Express 2HMG-mCherry in E. coli

    Depositor

    Kyubong Jo

    Article

    Lee et al RSC Adv, 2016, 6, 46291-46298

    Insert

    2HMG-mCherry
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p414GPD-3xFLAG-Nop4 WT

    Plasmid

    #169261

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 WT (NOP4 Budding Yeast)
    

    Tags

    3xFLAG

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_VAV3-1/2

    Plasmid

    #91528

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-1/2 (VAV3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103_SORBS3-1/3

    Plasmid

    #91534

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS3-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS3-1/3 (SORBS3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_VAV3-2/2

    Plasmid

    #91524

    Purpose

    Protein expression and purification of human SH3 domain construct VAV3-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV3-2/2 (VAV3 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHH0103_SH3YL1-1/1

    Plasmid

    #91485

    Purpose

    Protein expression and purification of human SH3 domain construct SH3YL1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3YL1-1/1 (SH3YL1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge