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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,859 results for: NSI

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Showing: 3841 - 3860 of 22859 results

  1. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  2. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  3. C51m

    Plasmid
    #120975
    Purpose
    MoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfYFP (has BbsI site)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120975-plasmid-map-sequence-id-236029

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  4. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

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  5. pBP-P_nahR-PSalTTC

    Plasmid
    #204074
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    nahR repressor and PSalTTC sodium salicylate-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    204074-plasmid-map-sequence-id-437326

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  6. pTU2-a_Sv5KA_amilCP

    Plasmid
    #204050
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var5 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    204050-plasmid-map-sequence-id-437162

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  7. pBP-T_BBa-B1006-U10

    Plasmid
    #204122
    Purpose
    Type 0 terminator part
    Depositor
    Mikhail Shapiro
    Insert
    BBa-B1006-U10 strong terminator
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204122-plasmid-map-sequence-id-418253

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  8. pTU2-a_Sv3KA_amilCP

    Plasmid
    #204052
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var3 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204052-plasmid-map-sequence-id-418172

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  9. pTU2-a_Sv6KA_amilCP

    Plasmid
    #204049
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var6 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204049-plasmid-map-sequence-id-418169

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  10. pTU2-a_Sv7KA_amilCP

    Plasmid
    #204048
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var7 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204048-plasmid-map-sequence-id-418164

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  11. pBP-P_luxR-PLuxB

    Plasmid
    #204073
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    luxR-PLuxB repressor and PLuxB 3OC6-AHL-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204073-plasmid-map-sequence-id-413889

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  12. pTU2-a_Sv4KA_amilCP

    Plasmid
    #204051
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var4 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204051-plasmid-map-sequence-id-413880

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  13. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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  14. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  15. U17m

    Plasmid
    #121023
    Purpose
    MoClo golden gate assembly BC part for Bujard RBS (strong RBS from https://doi.org/10.1093/nar/25.6.1203). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Bujard RBS
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121023-plasmid-map-sequence-id-245898

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  16. U19m

    Plasmid
    #121024
    Purpose
    MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121024-plasmid-map-sequence-id-238066

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  17. C85m

    Plasmid
    #120996
    Purpose
    MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma X (ECF20_992)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120996-plasmid-map-sequence-id-236125

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  18. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  19. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

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  20. C21m

    Plasmid
    #120962
    Purpose
    MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    MG-apt
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120962-plasmid-map-sequence-id-238043

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Showing: 3841 - 3860 of 22859 results