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Plasmid#135223PurposeMammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
Tags6xHis-HA-EYFPExpressionMammalianAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniABEmax (pRZ900)
Plasmid#131311PurposeCMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NYESO alpha into TRAC HDRT Source (pTR 223)
Plasmid#112023PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alphaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNYESO alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-roGFP1-mApple
Plasmid#107218PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertroGFP1-mApple
Tags4xCox8ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-B2AR-APEX
Plasmid#96848PurposeFor expression of B2AR-APEX in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB2AR-APEX (ADRB2 Human)
ExpressionMammalianAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
Plasmid#79614PurposeExpresses dCas9-SH3 and PmCDA1-SHL in yeast cellsDepositorPublicationInsertsSpCas9
PmCDA1
TagsSH3 domain and SHLExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1 and pGal10Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-H2B-C-10
Plasmid#58102PurposeLocalization: Nucleus/Histones, Excitation: 554, Emission: 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B (H2BC11 Human)
TagstdTomatoExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTurquoise-H2B-6
Plasmid#55557PurposeLocalization: Nucleus/Histones, Excitation: 434, Emission: 474DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B (H2BC11 Human)
TagsmTurquoiseExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 7, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
ECFP-H2B-6
Plasmid#55344PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B (H2BC11 Human)
TagsECFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
mCherry-H2B-18
Plasmid#55055PurposeLocalization: Nucleus/Histones, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B (H2BC11 Human)
TagsmCherryExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-H2B-C-10
Plasmid#55057PurposeLocalization: Nucleus/Histones, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B (H2BC11 Human)
TagsmCherryExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB CAG-sfGFP-T2A-iCreERT2
Plasmid#177309PurposeCAG-sfGFP-T2A-iCre-ERT2 piggyBac transposonDepositorInsertsfGFP-T2A-iCreERT2
UseCre/Lox; PiggybacExpressionMammalianAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS12: pDonor(AvCAST)
Plasmid#168145PurposeAvCAST donor harboring non-functional cargo.DepositorInsertsAvCAST right end (RE)
AvCAST left end (LE)
ExpressionBacterialAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-eN3x9
Plasmid#210706PurposeThis plasmid express EF1a promoter driven MCP-eN3x9 transactivation moduleDepositorInsertMCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
UseLentiviralTagsEGFPExpressionMammalianMutationN55K in MCPPromoterEF1aAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA
Plasmid#197027PurposeVector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized enRhCas12f1
ExpressionMammalianMutationL270RPromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3758 : CMVd3-HsIRE1-HaloTag
Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERN1 (ERN1 Synthetic, Human)
ExpressionMammalianAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA
Plasmid#193968PurposeEncodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthPol eta (POLH Human)
ExpressionMammalianPromoterCAGAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso C
Plasmid#137721PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoformDepositorInsertBRD4 short isoform (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterTight TRE promoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
Plasmid#172514PurposeEncodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoterDepositorInsertpAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector
UseAAVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only