164,811 results

1. PEmax-mRNA

   Plasmid

   #204472

   Purpose

   plasmid for PEmax mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

   Insert

   Prime editor
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Pur-CAG-hM3Dq-mCherry

   Plasmid

   #80948

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM3Dq-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM3Dq-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. IKK1-EYFP

   Plasmid

   #111206

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Insert

   IKKalpha (CHUK Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA63_Helper

   Plasmid

   #190432

   Purpose

   M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   M13 genes: I-XI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-hChR2(H134R)-EYFP (AAV5)

   Viral Prep

   #26973-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   EYFP

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. AAVS1-Pur-CAG-hM4Di-mCherry

   Plasmid

   #80947

   Purpose

   human AAVS1 site targeting donor plasmid for knock-in hM4Di-mCherry expression cassette driven by CAG promoter

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2016 Jun 2;18(6):817-26.

   Insert

   hM4Di-mCherry
   

   Use

   Tags

   mcherry fusion

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-hDcp2

   Plasmid

   #72214

   Purpose

   bacterial expression of His-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-Ikkb-FLAG(K44A)

   Plasmid

   #23299

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   Ikkbeta (IKBKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K44A

   Promoter

   Available since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

10. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Salsa-BC1-P1A1

    Plasmid

    #202842

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. RNA-binding protein pooled CRISPR knockout library

    Pooled Library

    #141438

    Purpose

    CRISPR knockout library targets RBPs with 10 sgRNAs/gene

    Depositor

    Eugene Yeo

    Article

    Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pT7-EGFP-C1-HsEDC4_S

    Plasmid

    #147455

    Purpose

    Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

    Depositor

    Elisa Izaurralde

    Article

    Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

    Insert

    HsEDC4 (EDC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKrox24(2xD-E_inD)Luc

    Plasmid

    #200109

    Purpose

    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element in front of EGR1 promoter…

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.1 myc-Rab10

    Plasmid

    #208366

    Purpose

    Mammalian expression of myc-tagged human Rab10

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10 (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. F5-bio-His

    Plasmid

    #53426

    Purpose

    Expresses full-length Coagulation factor V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F5 (F5 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human Epigenetic Knockout Library

    Pooled Library

    #162256

    Purpose

    Lentiviral CRISPR library targets human epigenetic genes

    Depositor

    Kivanc Birsoy

    Article

    Williams et al Cell Metab. 2020 Apr 7;31(4):852-861.e6.

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

18. 2xmyc-RAB10

    Plasmid

    #164631

    Purpose

    Expresses RAB10 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    Ras-related protein Rab-10 (RAB10 Human)
    

    Use

    Tags

    2xmyc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-116:PCNA-mEGFP

    Plasmid

    #170818

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA

    Depositor

    Allen Institute for Cell Science

    Insert

    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    June 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pDONR223-PRKAG3

    Plasmid

    #23549

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only