We narrowed to 6,482 results for: nls
-
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - NKX2.5-nEGFP
Plasmid#209287Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locusDepositorInsertEGFP-nls
UseKnockin donor vectorAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR1056
Plasmid#71824PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
EZ-L545
Plasmid#173024PurposeEncodes OptoEXP2 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1394
Plasmid#129522PurposepDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
EZ-L580
Plasmid#173023PurposeEncodes OptoAMP4 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastMutationA79QAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
M-SPcas
Plasmid#48668PurposeMammalian S. pyogenes Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSPromoterCMVAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
rtTA and TagBFP cassettes
Plasmid#62452PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMV::NLS-TagBFPx3-bGHpA
CMV::rtTA3-bGHpA
UseSynthetic BiologyAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)
Plasmid#174140PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…PromoterCMV and T7Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D.R
Plasmid#51510PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSPromoter2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK Smad3NL Flag
Plasmid#14828DepositorAvailable SinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCNPpo6-H98A
Plasmid#46087DepositorInsertPpoI H98A
TagsNLSExpressionMammalianMutationcatalytically inactive H98A variantPromoterCMVAvailable SinceJune 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZLSLD.R
Plasmid#51511PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSPromoter2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable SinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL1166
Plasmid#129520PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtinGW
Plasmid#133377PurposePiggybac vector for constitutive expressionDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK047
Plasmid#171319PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed-NLS fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only