We narrowed to 15,153 results for: RING;

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pET28-His10Smt3-gamma-toxin

Plasmid

#197000

Purpose

Bacterial expression plasmid for gamma toxin tRNA endonuclease

Depositor

Dragony Fu

Article

Lentini et al RNA. 2018 May;24(5):749-758. doi: 10.126

Insert

Gamma toxin

Tags

His10-Smt3

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-UXPS

Plasmid

#172138

Purpose

bacteria expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-BFP

Plasmid

#32639

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

BFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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miR-124-5 promoter GFP

Plasmid

#20792

Depositor

David Bartel

Article

Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

Insert

mir-124 (mir124-5 Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

Plasmid

#61255

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDW1775

Plasmid

#13456

Purpose

Plant ZFN expression vector.

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

AcV5

Expression

Plant

Available Since

Dec. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-P2A-strepII-PPPCB

Plasmid

#67923

Purpose

Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1E

Plasmid

#160442

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pEntr_PE2

Plasmid

#149548

Purpose

Gateway entry clone with PE2 enzyme (with stop codon)

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR; Gateway shuttle vector

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Promoter

vasa

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only
pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pRVV661

Plasmid

#108282

Purpose

Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

Insert

PhiC31 attP sites

Use

CRISPR

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS09

Plasmid

#67160

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS09

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

L414T within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pJG/ Inducible ALPHA MHC

Plasmid

#55792

Purpose

transgene expression driven by inducible ALPHA MHC promoter

Depositor

Jeffrey Robbins

Article

Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

Type

Empty backbone

Expression

Mammalian

Promoter

Inducible ALPHA MHC

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pJKR-H-ttgR

Plasmid

#62565

Purpose

Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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