We narrowed to 6,041 results for: SUP

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LENTICRISPR-UCK2_sgRNA2

Plasmid

#201633

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK2 (UCK2 Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K208D

Plasmid

#202785

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K208D

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-C264A

Plasmid

#202778

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

C264A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-R265A

Plasmid

#202783

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

R265A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H266A

Plasmid

#202784

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H266A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-RPE_sgRNA2

Plasmid

#201619

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

RPE (RPE Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI-UPP2-3xFLAG

Plasmid

#201645

Purpose

Over-expression of 3xFLAG-tagged human UPP2

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP2 (UPP2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-RPE_sgRNA1

Plasmid

#201618

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

RPE (RPE Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA2

Plasmid

#201629

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UCK1_sgRNA1

Plasmid

#201630

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK1 (UCK1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UCK1_sgRNA2

Plasmid

#201631

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK1 (UCK1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UCK2_sgRNA1

Plasmid

#201632

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UCK2 (UCK2 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5-toxic3

Plasmid

#188625

Purpose

pSC5GGv2 with HindII Gene Insert

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Insert

HindII

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

ACT1 intron insert

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Δ1-198)

Plasmid

#186222

Purpose

For expression of ATG13(Δ1-198) tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deleted amino acids 1 - 198

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

Plasmid

#198037

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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P2061

Plasmid

#186299

Purpose

Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

Depositor

Joerg Stelling

Article

Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

Inserts

LexA-HBD-B112
Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

2xLexop-minimalCyc1 and PACT1

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_591

Plasmid

#160653

Purpose

Expresses OLE1 under the control of the yeast Sup35 promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

YGL055W (OLE1 Budding Yeast)

Expression

Yeast

Promoter

Sup35 promoter

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-WT

Plasmid

#190094

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR Wild Type (ABHD2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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