We narrowed to 4,240 results for: erf

1. pdCas9-GFP

   Plasmid

   #181906

   Purpose

   Fluorescently tagged dead Cas9

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-CAG-IRF4-FLAG-IRES-GFP

   Plasmid

   #107389

   Purpose

   Lentiviral expression of IRF4-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH0006

   Plasmid

   #135448

   Purpose

   Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

   Depositor

   Jonathan Weissman, Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   dCas9-BFP-KRAB
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

   Plasmid

   #207953

   Purpose

   nuclease inactive control for TRF1FokI WT

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI (D450A)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

   Plasmid

   #207832

   Purpose

   telomere specific nuclease to create double-strand breaks at telomeres

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-dCas9-T2A-GFP

   Plasmid

   #193781

   Purpose

   Tricistronic expression of dCas9-EGFP-BSD

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   dead Cas9
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with existing Cas9 promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-dL5**-mCER-TRF1

    Plasmid

    #168176

    Purpose

    Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

    Depositor

    Patricia Opresko

    Article

    Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    FAP (dL5**) and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW-TetO-Irf7

    Plasmid

    #178445

    Purpose

    doxycycline-inducible expression of Mouse Irf7 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Irf7 (Irf7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-UCOE-SFFV-Zim3-dCas9-BFP

    Plasmid

    #188776

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid

    #60866

    Purpose

    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    CohIII
    CBM
    

    Use

    Tags

    6xHIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Cysteine 63 to Serine in CBM module

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid

    #60865

    Purpose

    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    Xyl
    Xmod-DocIII
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Threonine 120 to Cysteine in Xylanase domain

    Promoter

    T7

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139094

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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