We narrowed to 13,186 results for: Mpl

1. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

   Plasmid

   #199356

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-C-8His-Flag

   Plasmid

   #157678

   Purpose

   C-terminal tagging vector

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   8His-Flag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. F-Cdk8

   Plasmid

   #15366

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

   Insert

   CDK8 (CDK8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pShuttle-mCherry-EB1-C

   Plasmid

   #37829

   Depositor

   Torsten Wittmann

   Article

   Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Adenoviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   aa165-268 only

   Promoter

   CMV

   Available since

   Aug. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-3xFLAG-WPRE

   Plasmid

   #127862

   Purpose

   pAAV plasmid expressing 3xFLAG under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   3xFLAG
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-P26

   Plasmid

   #120037

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mAID-Clover
   

   Use

   Pcr template

   Tags

   mAID-Clover

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN mCherry-TAX1BP1(ΔUBD)

   Plasmid

   #119689

   Purpose

   For mammalian expression of TAX1BP1 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   UBD deletion (1-755)

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_hMed25

   Plasmid

   #206958

   Purpose

   The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

   Depositor

   Reza Zolfaghari, Catharine Ross

   Insert

   Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 HA Sam68 deltaKH

   Plasmid

   #17688

   Depositor

   David Shalloway

   Article

   Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

   Insert

   Sam68 (Khdrbs1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 157-256 deleted (KH domain)

   Promoter

   Available since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pCk2_spacer

    Plasmid

    #136072

    Purpose

    Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. PROM5_35Sx2

    Plasmid

    #136112

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35Sx2 (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. PROM5_35S

    Plasmid

    #136111

    Purpose

    Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_35S (CaMV)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. modRNAc0-Cas9

    Plasmid

    #170180

    Purpose

    IVT template plasmid for making modified Cas9 RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-TO-Sp dCas9-mVenusN

    Plasmid

    #191396

    Purpose

    SpdCas9 fused to N-terminal part of mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. YSBE 508 : pNEO_GFP

    Plasmid

    #92081

    Purpose

    Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection

    Depositor

    Yong-Sun Bahn

    Article

    So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

    Insert

    GFP
    

    Use

    tagging proteins in cryptococcus neoforman

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSPlit-OC-hDib1

    Plasmid

    #58789

    Purpose

    Express hDib1-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    hDib1 (TXNL4A Human)
    

    Use

    Tags

    Flag and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

17. LAMP1-ABKAR

    Plasmid

    #65068

    Purpose

    Expression of ABKAR biosensor targeted to the lysozome

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    ABKAR biosensor
    

    Use

    Tags

    Lamp1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3 Flag-MBD1-14-mVenusC

    Plasmid

    #191393

    Purpose

    MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. L1_KanR-Ck1

    Plasmid

    #136123

    Purpose

    L1 in position 1 (next to LB in L2 construct), for KanR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpEF1a:nptII_3t35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCk1_spacer

    Plasmid

    #136071

    Purpose

    Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge