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pDEST17-UbE2M

Plasmid

#15798

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2M (UBE2M Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
kiCAP-AAV-MyoAAV4A

Plasmid

#224448

Purpose

Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYNSL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s ONE-GO

Plasmid

#189731

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-s ONE-GO
GNAS (GNAS Human)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PEmax-mRNA

Plasmid

#204472

Purpose

plasmid for PEmax mRNA IVT

Depositor

Scot Wolfe

Article

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Insert

Prime editor

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-bARGSer-AxeTxe

Plasmid

#192473

Purpose

Second-generation bacterial acoustic reporter gene derived from Serratia

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Inserts

bARGSer
AxeTxe

Expression

Bacterial

Mutation

the gene Ser39006_001280 was deleted from the clu…

Promoter

Native promoter from Enterococcus faecium and pBAD

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-BFP

Plasmid

#188775

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LiLac

Plasmid

#184570

Purpose

Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

Depositor

Gary Yellen

Article

Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

Has Service

AAV8

Insert

LiLac fluorescent sensor of lactate

Use

AAV

Tags

His7

Expression

Mammalian

Promoter

CAG

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pK18sB

Plasmid

#177838

Purpose

Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

Depositor

Gregg Beckham, Christopher Johnson

Article

Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

Type

Empty backbone

Use

Bacterial gene replacement

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Article

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-JIP4

Plasmid

#164620

Purpose

Expresses JIP4 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163058

Purpose

roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-SPI1

Plasmid

#162824

Purpose

Doxycycline-inducible overexpression of human SPI1

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet ON

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM238

Plasmid

#161515

Purpose

Neuronal expression of histamine-sensitive chloride channel for negative selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Psnt-1 | histamine | rpl-3 UTR]

Expression

Worm

Promoter

Psnt-1

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpG-P2A-GFP

Plasmid

#159980

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpG-P2A-GFP

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP14/10

Plasmid

#159613

Purpose

Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10

Depositor

Opher Gileadi

Article

Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650

Inserts

Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
Covid-SARS2 Nsp10

Tags

His6, TEV cleavage site and none

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

(Bicistronic) and T7 - LacO

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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