We narrowed to 11,256 results for: NSI

1. pAAV-Ef1a-Con/Foff 2.0-mCherry

   Plasmid

   #137133

   Purpose

   Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223-ZNF706

   Plasmid

   #88037

   Purpose

   Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF706 (ZNF706 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-SARS2-S-D614 (NC-flag)

   Plasmid

   #156418

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

   Plasmid

   #75115

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   deleted bromodomain

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pHR-MED1trunc-IDR-YFP-NLS

   Plasmid

   #145275

   Purpose

   Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MED1-IDR truncated (MED1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223-ZNF692

   Plasmid

   #88701

   Purpose

   Donor Vector containing ZNF692 transcription factor, part of the Human TFome Collection

   Depositor

   Marc Vidal

   Article

   Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

   Insert

   ZNF692 (ZNF692 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Last nucleotide of stop codon removed to allow fo…

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p3xFLAG CMV14 / BRAF-D594A

   Plasmid

   #131717

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D594A

   Promoter

   CMV

   Available since

   Nov. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTWIN1-His6-Ssp-Httex1-7Q

   Plasmid

   #84347

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal His6-tagged intein Ssp

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

   Plasmid

   #75122

   Purpose

   Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   3x FLAG-tag

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-DIO-GRAB_SST1.0

    Plasmid

    #208666

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcdna6.2- myc ULK1 4SA

    Plasmid

    #27631

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Promoter

    Available since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-N3/hArf6(WT)-EGFP

    Plasmid

    #79423

    Purpose

    Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3xFLAG CMV14 / BRAF-G469A

    Plasmid

    #131711

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    G469A

    Promoter

    CMV

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP Cep170 C-term (Nigg GG2)

    Plasmid

    #41151

    Depositor

    Erich Nigg

    Article

    Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

    Insert

    Cep170 (CEP170 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains C-terminal fragment (aa 755-1460, from X…

    Promoter

    CMV, T7

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pDONR207 SARS-CoV-2 NSP3 Cys857Ala

    Plasmid

    #154027

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP3
    

    Use

    Gateway-compatible entry vector, with mutagenized…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

    Plasmid

    #154029

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP3
    

    Use

    Gateway-compatible entry vector, with mutagenized…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. TVBB C-term-mTurquoise2-Puro

    Plasmid

    #169224

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223 SARS-CoV-2 N_nostop

    Plasmid

    #152987

    Purpose

    Gateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    N
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pHAGE2-TetO-hSOX2

    Plasmid

    #206388

    Purpose

    Expresses human SOX2 in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

    Plasmid

    #171963

    Purpose

    Donor plasmid for CLTX T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a-tm CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid

    #159364

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

    Depositor

    Michael Farzan, Hyeryun Choe

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-HOXD13-IDR-mCherry-CRY2-NLS

    Plasmid

    #145260

    Purpose

    Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Ef1a-Coff/Fon-oScarlet

    Plasmid

    #137138

    Purpose

    Intersectional viral expression of oScarlet in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-45b-HOXD13(+10A)-IDR-mCherry

    Plasmid

    #145282

    Purpose

    Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+10A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21a-Med25-VBD-6His

    Plasmid

    #64773

    Purpose

    Bacterial expression of His-tagged Med25-VBD

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VBD (MED25 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insert is 394-543 only

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3/hArf3(WT)-mCherry

    Plasmid

    #79420

    Purpose

    Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf3 (ARF3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-Myc-PGK-Puro-IRES-GFP

    Plasmid

    #75124

    Purpose

    Retroviral expression plasmid encoding wild type murine Myc

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pAAV-EGFP.T2A.NCLX

    Plasmid

    #181873

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    

    Use

    AAV and Adenoviral

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    synthetic hybrid CAG promoter and synthetic hybri…

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. VBS mutant of alpha catenin sensor

    Plasmid

    #71710

    Purpose

    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin ΔVBS FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted amino acid residues 316-405 in α -catenin…

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUW-TetO-Eomes

    Plasmid

    #128825

    Purpose

    dox-dependent expression of mouse Eomes

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Shorter isoform

    Promoter

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223-ZNF770

    Plasmid

    #88562

    Purpose

    Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF770 (ZNF770 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. FUW-TetO-Gata3

    Plasmid

    #128824

    Purpose

    dox-dependent expression of mouse Gata3

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP VAMP7 (102-220)

    Plasmid

    #42314

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ1-102

    Promoter

    CMV

    Available since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pXPR_BRD003 sgKEAP1-1

    Plasmid

    #74985

    Purpose

    sgKEAP1-1, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGL2-Mcl-1 mut

    Plasmid

    #19133

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

    Insert

    Mcl-1 promoter (MCL1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    NFkB binding-site mutant, the sequence GGGGTCTTCC…

    Promoter

    Available since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid

    #159363

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

    Depositor

    Michael Farzan, Hyeryun Choe

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Use

    Tags

    Flag and Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AAVS1-Puro CAG-CLTX CD32a ITAM CAR

    Plasmid

    #171964

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

    Plasmid

    #171965

    Purpose

    Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

    Insert

    Synthetic CLTX CD32a ITAM CD3z CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Michael Farzan, Hyeryun Choe

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR223 SARS-CoV2 TEV-E

    Plasmid

    #154418

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of e…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    July 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge