We narrowed to 5,560 results for: SPI

1. PTEF1-yEGFPCLN2PEST-pRS406

   Plasmid

   #64406

   Purpose

   TEF promoter driven GFP expression

   Depositor

   Claudia Vickers

   Article

   Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

   Insert

   TEF1 promoter (TEF1 Budding Yeast)
   

   Use

   Tags

   yEGFPCLN2PEST (destabilized yeast EGFP)

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS183

   Plasmid

   #134142

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT005

   Plasmid

   #171632

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-SARS-CoV-2-E

   Plasmid

   #215814

   Purpose

   Expresses SARS-CoV-2 Envelope protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Envelope (E )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SARS-CoV-2-M

   Plasmid

   #215813

   Purpose

   Expresses SARS-CoV-2 Membrane protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Membrane (M )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FEMP

   Plasmid

   #191973

   Purpose

   FRET-based indicator of ER-mitochondria proximity (FEMP)

   Depositor

   Luca Scorrano

   Article

   Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

   Insert

   AkapYFP-Tav2a-CFP-sac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEK36

   Plasmid

   #210598

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
   

   Use

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac-His-MBP-SCov2-nsp12

   Plasmid

   #154759

   Purpose

   Expression of SARS-CoV-2 nsp12

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedron

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SDHC-pHLuorin-sems

    Plasmid

    #107767

    Purpose

    labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit C of SDH fused to pHluorin (SDHC Human)
    

    Use

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. NP-11-64-1

    Plasmid

    #234359

    Purpose

    Expressing rubisco and prk in Δrpi

    Depositor

    David Savage

    Article

    Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

    Insert

    R.rubrum rubisco
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175019

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    D/bovine/Nebraska/9-5/2012
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-IRES-GFP-human-Caspase-1

    Plasmid

    #183359

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-5' MS2-CircRNA

    Plasmid

    #213750

    Purpose

    For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-Lhx3

    Plasmid

    #32927

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Lhx3 (Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pMXS-IRES-Blast coUGP2

    Plasmid

    #211531

    Purpose

    Expresses codon optimized UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UGP2 (UGP2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Phi3-GCaMP6f

    Plasmid

    #106298

    Purpose

    CMV driven GFP based calcium (Ca2+) reporter

    Depositor

    Sanford Simon

    Article

    Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-MyosinVb-Ctail

    Plasmid

    #110170

    Purpose

    Dominant negative effect by competing with MyosinVb C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1262-1670

    Promoter

    CMV

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-Ngn2

    Plasmid

    #32928

    Purpose

    Retroviral expression of mouse Ngn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTK88_GFP-Anillin

    Plasmid

    #46354

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    Anillin (ANLN Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    a1854 is changed to c to disrupt XhoI site withou…

    Promoter

    pBABE

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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