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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,812 results for: cloning

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Showing: 3901 - 3920 of 63812 results

  2. His6-pAG-RTase

    Plasmid
    #214932
    Purpose
    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems
    Depositor
    Chuan He
    Publication
    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s
    Insert
    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…
    Promoter
    T7 promoter
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214932-plasmid-map-sequence-id-426479

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  7. pJR100

    Plasmid
    #187240
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187240-plasmid-map-sequence-id-374768

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  9. pAAV-CAG-LiLac

    Plasmid
    #184570
    Purpose
    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV
    Depositor
    Gary Yellen
    Publication
    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:
    Has service
    AAV8
    Insert
    LiLac fluorescent sensor of lactate
    Use
    AAV
    Tags
    His7
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184570-plasmid-map-sequence-id-490399

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  11. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

    Plasmid
    #172910
    Purpose
    Mammalian expression of voltage sensor; soma localization targeting sequence
    Depositor
    GENIE Project
    Publication
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Voltron2-ST
    Tags
    soma localization targeting sequence
    Expression
    Mammalian
    Mutation
    A122D
    Promoter
    CAG
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172910-plasmid-map-sequence-id-341196

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  13. UBC plasmid

    Plasmid
    #169746
    Purpose
    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
    Depositor
    Francois St-Pierre
    Publication
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    UBC
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169746-plasmid-map-sequence-id-334556

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  15. CGBE1-NG (pBM1246)

    Plasmid
    #140256
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140256-plasmid-map-sequence-id-291778

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Showing: 3901 - 3920 of 63812 results