We narrowed to 14,399 results for: nts

1. pICH45244

   Plasmid

   #50277

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter + 5' UTR, RbcS3B (AT5g38410, A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRATIO2251

   Plasmid

   #141305

   Purpose

   Gateway cloning compatible ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Use

   Tags

   Firefly Luciferase or LUC2 and mScarlet-I

   Expression

   Plant

   Mutation

   Promoter

   UBQ10 promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF204

   Plasmid

   #121656

   Purpose

   U6-sgRNA EFS-Cas9-wt-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE3

   Plasmid

   #79442

   Purpose

   recipient plasmid [multiplexing]; pPcUbi:Cas9, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGE64

   Plasmid

   #79446

   Purpose

   recipient plasmid [single nuclease]; pPcUbi:Cas9, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE1

   Plasmid

   #79440

   Purpose

   recipient plasmid [multiplexing]; p35S:Cas9, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   ccdB (ccdB(letD) )
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald-PCNA-19-NLS-4

   Plasmid

   #54222

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

   Insert

   PCNA-19 NLS (PCNA Human)
   

   Use

   Tags

   SV40NLS and mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pTRANS_130

   Plasmid

   #91204

   Purpose

   Transformation, Type: non-T-DNA, Plant Selection: 2x35S:bar, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pc3XB-ZF5

   Plasmid

   #13104

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   ZF5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRANS_202

    Plasmid

    #91106

    Purpose

    Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pc3XB-ZF10

    Plasmid

    #13109

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDH5

    Plasmid

    #83775

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; his selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

    Promoter

    Available since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDS66

    Plasmid

    #48364

    Purpose

    3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDS67

    Plasmid

    #48365

    Purpose

    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPZP FLIPmal-25u

    Plasmid

    #14476

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPmal
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    W230A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. GgVcl 1-1066 FRET tail probe 2

    Plasmid

    #46282

    Depositor

    Susan Craig

    Article

    Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-9

    Insert

    Vcl 1-1066 + mCitrine (VCL Chicken)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    mCitrine (and linker) inserted after aa883

    Promoter

    CMV

    Available since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pc3XB-ZF64

    Plasmid

    #13200

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T1 human APC-B,C (1133–1189)

    Plasmid

    #132630

    Purpose

    expresses human APC-B,C (1133–1189) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

    Insert

    APC-B,C (1133–1189)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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