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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,299 results for: sgRNA

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Showing: 3921 - 3940 of 17299 results

  6. pSCBE3-NG-HF1-Hypa

    Plasmid
    #218162
    Purpose
    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218162-plasmid-map-sequence-id-433200

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  16. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid
    #157981
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Publication
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157981-plasmid-map-sequence-id-309444

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  17. pSCIP-hTRAC

    Plasmid
    #154094
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA and BAIT targeting human TRAC locus
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154094-plasmid-map-sequence-id-307107

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  19. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2-sgRNA8 (BACH2 S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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Showing: 3921 - 3940 of 17299 results