We narrowed to 17,657 results for: NOL

1. pColE1_sgRNA_NT_AmpR

   Plasmid

   #119144

   Purpose

   Encodes non-targeting sgRNA

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   non-targeting sgRNA
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC2483 (pSpy1C)

   Plasmid

   #220248

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TEVSH

   Plasmid

   #125194

   Purpose

   His-tagged TEV protease with solubility increasing mutations

   Depositor

   Helena Berglund

   Article

   van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

   Insert

   TEV_SH
   

   Use

   Tags

   V5 His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB591

   Plasmid

   #141096

   Purpose

   E. coli-Lactobacilli shuttle vector for recombineering template cloning

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p104_gRNA_Sp_CD3e

   Plasmid

   #213780

   Purpose

   Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sp gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pColE1_sgRNA_pos8_AmpR

   Plasmid

   #119151

   Purpose

   Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCM4.4

   Plasmid

   #217648

   Purpose

   an ermE*p-cas9-driven expression, as an alternative plasmid

   Depositor

   Huimin Zhao

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   Codon Optimized SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-His-CKM

   Plasmid

   #124134

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Creatine kinase (CKM Chicken)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p105_gRNA_Sa_CD40

    Plasmid

    #213781

    Purpose

    Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLH-sgRNA1-2XPP7

    Plasmid

    #75390

    Purpose

    sgRNA1-2XPP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDA303

    Plasmid

    #195199

    Purpose

    Overexpressing 6His-TetR in E. coli.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    TetR
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEZYmyc-His

    Plasmid

    #18701

    Purpose

    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC574

    Plasmid

    #127975

    Purpose

    AMA1 plasmid with hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    hygB (hygromycin resistance marker)
    

    Use

    Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans trpC promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi-GN

    Plasmid

    #60517

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMKV059

    Plasmid

    #133314

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and IPT

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc+, IPT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUbi10, 35S

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBR1k-GFPuv

    Plasmid

    #106382

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR1k-con-GFPuv

    Plasmid

    #106386

    Purpose

    Constitutive trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBB541

    Plasmid

    #27394

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    groESL (groL, groS E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMT406

    Plasmid

    #139384

    Purpose

    RPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    RPU standard
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    No promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMAL-c4X

    Plasmid

    #75288

    Purpose

    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL193

    Plasmid

    #180471

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Cas9

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pU6-Lb-crRNA

    Plasmid

    #78957

    Purpose

    Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSET-mSA-EGFP

    Plasmid

    #39862

    Depositor

    Sheldon Park

    Article

    Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

    Insert

    monomeric streptavidin fused to EGFP
    

    Use

    Tags

    6xHis, EGFP, and FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBb(RK2)1k-GFPuv

    Plasmid

    #106394

    Purpose

    IPTG-inducible trc promoter expressing gfpuv on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMT405

    Plasmid

    #139383

    Purpose

    LacZ, pNBU1 backbone with BsaI sites, AmpR, ErmR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    LacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    No promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PX851

    Plasmid

    #62883

    Purpose

    N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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