Addgene

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pcDNA3.2 mir-1-1 reporter cel-let-7

Plasmid

#46648

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-let-7

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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Khc73(1-387)-GFP

Plasmid

#31747

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Use

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-387

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
Khc73(1-432)-GFP

Plasmid

#31748

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Use

Tags

GFP

Expression

Mammalian

Mutation

Contains only aa 1-432

Promoter

Metallothionein

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only
GFP-Rab32

Plasmid

#31744

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab32 (Rab32 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
ptfLC3

Plasmid

#21074

Purpose

Mammalian expression of rat LC3 fused to mRFP and EGFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Use

Tags

cfSGFP2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCM184-ParB2::eGFP

Plasmid

#87252

Purpose

Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB2

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

pBAD/tetO

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCM189-ParB1::mCherry

Plasmid

#87253

Purpose

Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

ParB1

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pBAD/tetO

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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C413-E45: attB4-hr5-ie1p>-attB1r

Plasmid

#162930

Purpose

Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV ie1 promoter with hr5 enhancer

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer

Plasmid

#41584

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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hNICD3(3xFLAG)-pCDF1-MCS2-EF1-copGFP

Plasmid

#40640

Depositor

Brenda Lilly

Article

Zhao et al J Biol Chem. 2012 May 11;287(20):16111-2

Insert

NICD3 (NOTCH3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Article

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Use

Tags

HA and c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

tetO7 promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Article

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Use

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHrD-IRES-Fluc

Plasmid

#194250

Purpose

Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!

Depositor

Samson Jacob

Article

McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

human rDNA promoter and beginning of 5' ETS …

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mRuby3-2A-Cre

Plasmid

#203844

Purpose

Flp-dependent Cre and mRuby3 expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mRuby3-2A

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1

Plasmid

#1026

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

Plasmid

#203842

Purpose

Flp-dependent Cre and EGFP expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

EGFP-2A

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3C WT

Plasmid

#123095

Purpose

Expresses 3xFLAG-LC3C wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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T7 promoter + filler DNA reporter plasmid

Plasmid

#156454

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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T7 promoter + terminators reporter plasmid

Plasmid

#156456

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre

Plasmid

#203838

Purpose

Flp-dependent Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pJT9R

Plasmid

#98305

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TRPL3AcNES1>TRPL41B

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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mbYFPQS

Plasmid

#80742

Purpose

membrane-targeted biosensor for monitoring changes in intracellular chloride

Depositor

Susan Amara, Susan Ingram

Article

Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

Insert

YFPQS

Use

Tags

palmitoylation signal sequence of neuromodulin

Expression

Mammalian

Mutation

H148Q (CAC to CAG) and V163S (GTG to AGC).

Promoter

CMV

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only
pBI-CMV1-mir144-CMV2-mir1

Plasmid

#164634

Purpose

Express miR-144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

mir144 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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