We narrowed to 7,663 results for: ski

1. pDF0317 HvsCas7-11 mammalian expression

   Plasmid

   #172511

   Purpose

   Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   HvsCas7-11 mammalian expression
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRY2(535, L348F)-mCherry

   Plasmid

   #75372

   Purpose

   Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 residues 1-535 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   L348F

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

   Plasmid

   #194678

   Purpose

   CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   CHRM3_cpHalo∆
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA SF-HERC2 F4

   Plasmid

   #55787

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. CRY2PHR(W349R)-mCherry

   Plasmid

   #75370

   Purpose

   Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 residues 1-498 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W349R

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

   Plasmid

   #138145

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NLS

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPV-EF1a-mRxxFP1(AfeI)-iP-A

   Plasmid

   #204625

   Purpose

   Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gal4BD-CRY2(535)

   Plasmid

   #75369

   Purpose

   Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Tags

   Gal4BD

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA SF-HERC2 F5

   Plasmid

   #55788

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
   

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

    Plasmid

    #169328

    Purpose

    Expression of HaloTag9 located at the microtubules of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag9 (CEP41 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT/TO_TOMM20_HaloTag9

    Plasmid

    #169335

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hsyn-DIO-GCaMP6s

    Plasmid

    #184284

    Purpose

    Expresses GCaMP6s in genetically defined neurons expressing Cre recombinase

    Depositor

    William Wisden

    Article

    Yu et al Nat Neurosci. 2019 Jan;22(1):106-119. do

    Insert

    CCaMP6s
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    human synaptophysin

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Tags

    SNAP-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

    Plasmid

    #194685

    Purpose

    CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL1-SpCas9

    Plasmid

    #117051

    Purpose

    In vitro expression of S. pyogenes Cas9 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    S. pyogenes Cas9
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MET7A-CD4d3+4-bio

    Plasmid

    #73127

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding MET7A with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    MET7A (METTL7A Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194687

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P shSOD1

    Plasmid

    #102975

    Purpose

    Suppression of SOD1

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shSOD1 (SOD1 Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIP hGMCSF co

    Plasmid

    #74168

    Purpose

    Lentiviral vector for expression of human codon optimized GMCSF with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    GMCSF (CSF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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