We narrowed to 31,203 results for: uros

1. pGEX SYT9 C2B D330, 332N

   Plasmid

   #195705

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 253-344 of mouse SYT9 with D3…

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX SYT9 C2AB D197,199,330,332N

   Plasmid

   #195706

   Purpose

   Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes amino acids 104-386 of mouse SYT9 with D1…

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-flex-GFP-shRNA-scramble

   Plasmid

   #182503

   Purpose

   Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
   

   Use

   AAV, Cre/Lox, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-iCre-2A-venus

   Plasmid

   #182499

   Purpose

   Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   iCre-2A-Venus
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   fragment of histidine decarboxylase gene promoter…

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSynW SYT9 D197, 199N IRES GFP

   Plasmid

   #195701

   Purpose

   Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full-length mouse SYT9 with D197, 199N mutations

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFSynW SYT9 D330, 332N IRES GFP

   Plasmid

   #195702

   Purpose

   Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Syt9 (Syt5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full-length mouse SYT9 with D330, 332N mutations

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti SYN-SVI-DIO-dCas9-VPR

   Plasmid

   #164576

   Purpose

   Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_TRET_Ngn2‐2A‐Ngn1

   Plasmid

   #61471

   Purpose

   3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Inserts

   Ngn2 (Neurog2 Mouse)
   Neurog1 (Neurog1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-DIO-trkB.DN-mCherry

   Plasmid

   #121502

   Purpose

   Expresses trkB.T1 fused with mCherry in a cre dependent manner.

   Depositor

   Marie Carlén

   Article

   Guyon et al J Neurosci. 2021 Feb 15. pii: JNEUROSCI.

   Insert

   trkB.T1 (Ntrk2 Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFSynW SYT9 D197, 199, 330, 332N IRES GFP

    Plasmid

    #195703

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D197, 199, 330, 332N …

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid

    #170725

    Purpose

    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

    Depositor

    George Haughn

    Article

    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

    Insert

    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    

    Use

    Gateway donor vector / entry clone

    Tags

    Citrine tag (714bp)

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR207-MUM2sp-ADPG2

    Plasmid

    #170723

    Purpose

    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

    Depositor

    George Haughn

    Article

    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

    Insert

    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    

    Use

    Gateway donor vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

    Plasmid

    #160069

    Purpose

    Conditionally overexpress collybistin (CB2SH3-) and mCitrine

    Depositor

    Angel de Blas

    Article

    George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    AAV

    Tags

    HA- in CB2SH3

    Expression

    Mutation

    Promoter

    human Synapsin promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-tetO-NGN2

    Plasmid

    #197090

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2 (NEUROG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

    Plasmid

    #122981

    Purpose

    AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Synthetic, Rat)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

    Plasmid

    #122982

    Purpose

    AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    mCh-IRES-BoNT/B(1-146)-iLID
    

    Use

    AAV

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    iLID contains long lived V416I mutation

    Promoter

    human synapsin

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-tetO-NB

    Plasmid

    #197092

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid

    #122985

    Purpose

    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    

    Use

    AAV

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    SspB contains A58V,R73Q "milli" mutatio…

    Promoter

    human synapsin

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Anti-VAPA/B [N479/107R]

    Plasmid

    #188163

    Purpose

    Mammalian Expression Plasmid of anti-VAPA and -VAPB (Rat). Derived from hybridoma N479/107.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VAPA and -VAPB (Rattus norvegicus) recombinant mouse monoclonal antibody (Vapa, Vapb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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