We narrowed to 22,109 results for: ESC

1. DmU6 3p3 GFP LgRNA

   Plasmid

   #182207

   Purpose

   For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

   Depositor

   Craig Montell

   Article

   Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

   Insert

   3XP3-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJB028

   Plasmid

   #65469

   Purpose

   pTrc plasmid expressing p14*-TEV

   Depositor

   Keith Tyo

   Article

   Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

   Insert

   p14*-TEV (TEVgp1 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Clover-Lifeact-7

   Plasmid

   #54551

   Purpose

   Localization: Actin, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

   Plasmid

   #162812

   Purpose

   STELLA plasmid for amber suppression of C-terminally labelled GFP

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   GFP(C-TAG)-Intein-DD
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   GFP(Opal247,Amber)

   Promoter

   EF1alpha

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-link-yoLSS-mKate2-CaURA3

   Plasmid

   #44886

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoLSS-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEos3.2-LaminA-C-18

   Plasmid

   #57463

   Purpose

   Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. Dronpa-Vinculin-23

   Plasmid

   #57308

   Purpose

   Localization: Focal Adhesions, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Article

   Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   Dronpa

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pYTSG

   Plasmid

   #159419

   Purpose

   Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   polyhistidine (poly-His)

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDF-EcAvs2

   Plasmid

   #188804

   Purpose

   Codon optimized EcAvs2 with C-terminal His tag

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-link-yomWasabi-Kan

    Plasmid

    #44945

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomWasabi
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD

    Plasmid

    #162813

    Purpose

    STELLA plasmid for amber suppression of C-terminally labelled NoBody

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    NoBody(C-TAG)-Intein-DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    NoBody(Opal69,Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-link-yomEos2-CaURA3

    Plasmid

    #44887

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mEos2 with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomEos2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-Chimeric_Butterfly_YR_1.0

    Plasmid

    #59801

    Purpose

    Genetically encoded fluorescent voltage indicator

    Depositor

    Thomas Knopfel

    Article

    Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

    Insert

    Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET22b_PfSSB

    Plasmid

    #103000

    Purpose

    The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB

    Depositor

    Martin Webb

    Article

    Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

    Insert

    PfSSB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Aurein1.2_(pos36GFP)-Cys

    Plasmid

    #71757

    Purpose

    Aurein1.2_+36GFP-Cys expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    Aurein1.2_(pos36_GFP)-Cys
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEB2-Katushka-9-5

    Plasmid

    #104011

    Purpose

    Plasmid encoding Katushka-9-5

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Katushka9-5
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (K6S, N21D, R32G, L…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mTurquoise2-UtrCH

    Plasmid

    #98824

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    UtrCH
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-link-yomKO2-Kan

    Plasmid

    #44958

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKO2 with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CD3delta-CFP

    Plasmid

    #128154

    Purpose

    Expression of CD3 delta

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Lorenz et al Nat Methods. 2006 Mar;3(3):205-10. doi:

    Insert

    CD3 Delta (Cd3d Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKB239

    Plasmid

    #170034

    Purpose

    Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Granulysin (GNLY Synthetic, Human, Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 3XFlag-pLPC

    Plasmid

    #73560

    Purpose

    vector encoding 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    3X-Flag
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pNIR-Fb2E7

    Plasmid

    #184681

    Purpose

    NIR-Fb to HIV gp41

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb2E7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBABE-puro-NLS-LSSmKate1

    Plasmid

    #31873

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Gag-delta-p6-mEGFP

    Plasmid

    #116890

    Purpose

    expresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    synthetic Gag missing the p6 domain
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Kohinoor-Actin/pcDNA3

    Plasmid

    #67776

    Purpose

    Actin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

26. P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

    Plasmid

    #70211

    Purpose

    1 Part of 4-part switch

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deCFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mEos3.2-MCPB1-N-18

    Plasmid

    #57472

    Purpose

    Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    MCPB1 (Capzb Mouse)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pSB1A3-AD009

    Plasmid

    #116850

    Purpose

    Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

    Depositor

    Friedrich Simmel

    Article

    Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

    Inserts

    AraC
    alpha-HL
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFA6a-link-yomRuby-SpHis5

    Plasmid

    #44843

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKS011

    Plasmid

    #65464

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    MBP-Ntag-mCherry
    

    Use

    Tags

    FLAG and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pl-TetO

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid

    #85398

    Purpose

    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Mutation

    S27A

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-D1

    Plasmid

    #37480

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1 second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pFA6a-link-yomApple-SpHis5

    Plasmid

    #44844

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomApple
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAS1_4x7SKPylT_EF1_Ub-*NoBody

    Plasmid

    #162807

    Purpose

    STELLA plasmid for amber suppression of N-terminally labelled NoBody

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-(N-TAG)NoBody
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    NoBody (Met1, Amber)

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p3s-Cas9-HF1

    Plasmid

    #104173

    Purpose

    Cas9-HF1 mammalian expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Insert

    Cas9-HF1
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    CMV

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV_hSyn_Ilmol

    Plasmid

    #120311

    Purpose

    Expresssion of Ilmol preferentially in neurons

    Depositor

    Bradley Baker

    Article

    Yi et al Sci Rep. 2018 Oct 12;8(1):15199. doi: 10

    Insert

    Ilmol
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBAD-iGECInano

    Plasmid

    #186189

    Purpose

    Expression of small FRET-based near-infrared genetically-encoded calcium indicator in bacteria

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

    Insert

    iGECInano
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBCC155

    Plasmid

    #74995

    Purpose

    CFP-based BiFC vector for transient expression of CC (155-238) in plants

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    CFP (155–239)/CC155

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBCN155

    Plasmid

    #74994

    Purpose

    CFP-based BiFC vector for transient expression of CN (1-155) in plants

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    CFP (1–155)/CN155

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFA6a-link-yoPA-mCherry-SpHis5

    Plasmid

    #44855

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPA-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge