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HcKan_P

Plasmid

#65314

Purpose

receiving vector of promoters for the construction of standard promoter parts in YeastFab work

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pUC57KAN-80XALFA

Plasmid

#233124

Purpose

80X ALFA tag multimer

Depositor

Steve Stowers

Insert

ALFA tag

Use

Unspecified

Mutation

None

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57KAN-20XALFA

Plasmid

#233112

Purpose

20X ALFA tag multimer

Depositor

Steve Stowers

Insert

ALFA tag

Use

Unspecified

Mutation

None

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available Since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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RS02060_pMQ30_KO_Kan

Plasmid

#185391

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS02060 upstream flanking homology region
RR42_RS02060 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS28935_pMQ30_KO_Kan

Plasmid

#185392

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS28935 upstream flanking homology region
RR42_RS28935 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18965_pMQ30_KO_Kan

Plasmid

#185394

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18965 upstream flanking homology region
RR42_RS18965 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS18970_pMQ30_KO_Kan

Plasmid

#185395

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18970 upstream flanking homology region
RR42_RS18970 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17060_pMQ30_KO_Kan

Plasmid

#185396

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17060 upstream flanking homology region
RR42_RS17060 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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RS17055_pMQ30_KO_Kan

Plasmid

#185398

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS17055 upstream flanking homology region
RR42_RS17055 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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attB-GT-mNeonGreen-linker::pUC-GW-Kan_mod

Plasmid

#244895

Purpose

Minicircle producer plasmid

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

mNeonGreen

Use

Unspecified

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel_CAG_RNeoStop_WPRE_bgh_pA

Plasmid

#97011

Purpose

Rosa26 targeting vector for Dre-responsive cassette

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR and Cre/Lox

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUTKan-3xHA-GFP-TOM5

Plasmid

#130674

Purpose

Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

Depositor

Andreas Weber

Article

Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

Insert

Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)

Tags

3xHA and sGFP

Expression

Plant

Promoter

Ubiquitin10

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-KanMX6-Purg1-3FLAG

Plasmid

#19354

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

urg1 promoter

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_TH16_00079081

Plasmid

#121372

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

TH16_00079081

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_TH16_00118381

Plasmid

#121374

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

TH16_00118381

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_TH16_00106651

Plasmid

#121373

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

TH16_00106651

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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