We narrowed to 38,918 results for: LAT;

1. TPC2(N257A)-mCherry

   Plasmid

   #135186

   Purpose

   Pore-dead mutant of TPC2, tagged on C terminus with mCherry.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TPC2 (Tpcn2 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Mutation of asparagine257 to alanine

   Promoter

   Available since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CSTF1

   Plasmid

   #155485

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CSTF1 (CSTF1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PiggyBac-FLAG-mProser1-IRES-Blast

   Plasmid

   #226172

   Purpose

   Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

   Insert

   Proline Serine Rich protein 1 (Proser1 Mouse)
   

   Use

   Tags

   2X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-STIM1-NN_neomycin

   Plasmid

   #65311

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC8 human IFN-gamma

   Plasmid

   #17600

   Depositor

   Howard Young

   Article

   Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

   Insert

   interferon-gamma cDNA (IFNG Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. SFRS16

   Plasmid

   #156009

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SFRS16 (CLASRP Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE-HA-Ku70-siR-WT

   Plasmid

   #82329

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tet

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIPZ-TIR1-HA

   Plasmid

   #172610

   Purpose

   Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   TIR1
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Tet/ON Minimal CMV promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NOTCH3-bio-His

   Plasmid

   #53343

   Purpose

   Expresses full-length Neurogenic locus notch homolog protein 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   NOTCH3 (NOTCH3 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid

    #127177

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mutation

    Promoter

    None

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TPC1(ΔN69)-mCherry

    Plasmid

    #135184

    Purpose

    TPC1 with truncated N-terminus, tagged with mCherry on C terminus.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC1 (Tpcn1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-69

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TPC2(ΔN49)-mCherry

    Plasmid

    #135185

    Purpose

    TPC2 with truncated N-terminus, tagged with mCherry on C terminus.

    Depositor

    Antony Galione

    Article

    Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

    Insert

    TPC2 (Tpcn2 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-49

    Promoter

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CLP1

    Plasmid

    #155462

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CLP1 (CLP1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-Neo-TetO-Cdk12_Untagged

    Plasmid

    #127178

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TetO Promoter

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FZD8

    Plasmid

    #155618

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    FZD8 (FZD8 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pINDUCER-21-RUNX1-P2A-ERG

    Plasmid

    #97045

    Purpose

    Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1, ETS-Related Gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MBP-CHC(1-1074)-His6

    Plasmid

    #100748

    Purpose

    Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CLTC (CLTC Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Residues 1-1074

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNAI-GAL4-CREB

    Plasmid

    #46769

    Purpose

    Expression of GAL4 fusion to wild type CREB

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    GAL4(1-147)-CREB (Creb1, GAL4 Budding Yeast, Rat)
    

    Use

    Tags

    GAL4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. ADARB1

    Plasmid

    #155396

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ADARB1 (ADARB1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CD69-His-bio

    Plasmid

    #52328

    Purpose

    Expresses full-length C-type lectin domain family 2 member C ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD69 (CD69 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge