Skip to main content
Addgene
Showing: 31 - 60 of 172 results

  2. hPAX7-3xHA-IRES-hrGFP (#400)

    Plasmid
    #78338
    Purpose
    Overexpression of human PAX7 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    PAX7 (PAX7 Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. hMYOD1-3xHA-IRES-hrGFP (#343)

    Plasmid
    #78327
    Purpose
    Overexpression of human MYOD1 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78327-plasmid-map-sequence-id-456542

    Enlarge

  5. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid
    #127239
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger2-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127239-plasmid-map-sequence-id-247086

    Enlarge

  6. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid
    #129394
    Purpose
    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    soma targeted ChRger2
    Use
    AAV and Cre/Lox
    Tags
    Kv2.1-TS-EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129394-plasmid-map-sequence-id-259728

    Enlarge

  8. hMYOG-3xHA-IRES-hrGFP (#324)

    Plasmid
    #78341
    Purpose
    Overexpression of human MYOG ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOG (MYOG Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. hMYF5-3xHA-IRES-hrGFP (#397)

    Plasmid
    #78331
    Purpose
    Overexpression of human MYF5 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYF5 (MYF5 Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV-CAG-DIO(ChRger1-TS-YFP)

    Plasmid
    #127245
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger1-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127245-plasmid-map-sequence-id-247234

    Enlarge

  14. pAAV-CAG-DIO(ChRger3-TS-YFP)

    Plasmid
    #127242
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger3-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127242-plasmid-map-sequence-id-247106

    Enlarge

  17. pCSIIpuro-NRG1-ScNeo

    Plasmid
    #209900
    Purpose
    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    NRG1-ScNeo (NRG1 Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209900-plasmid-map-sequence-id-416651

    Enlarge

  19. pPBbsr2-NRG1-ScNeo

    Plasmid
    #209911
    Purpose
    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    NRG1-ScNeo (NRG1 Human)
    Use
    Tags
    mNeonGreen and mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209911-plasmid-map-sequence-id-416696

    Enlarge

  20. pPBpuro-NRG1-ScNeo

    Plasmid
    #209906
    Purpose
    To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    NRG1-ScNeo (NRG1 Human)
    Use
    Tags
    mNeonGreen and mScarlet
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209906-plasmid-map-sequence-id-416689

    Enlarge

  21. pTol2CG NBT:NRGIIIa_R551Q:polyA

    Plasmid
    #193013
    Purpose
    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons
    Depositor
    William Talbot
    Article
    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi
    Inserts
    NBT
    NRG1 type IIIa R551Q (NRG1 Human)
    Use
    Transposon transgenesis
    Tags
    Expression
    Mammalian
    Mutation
    ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    193013-plasmid-map-sequence-id-379620

    Enlarge

  23. pDONR221-SLC48A1_STOP

    Plasmid
    #161322
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC48A1 (SLC48A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161322-plasmid-map-sequence-id-360556

    Enlarge

  24. pAAV-shMcu-1

    Plasmid
    #181868
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181868-plasmid-map-sequence-id-356032

    Enlarge

  25. pAAV-shMcu-2

    Plasmid
    #181867
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181867-plasmid-map-sequence-id-356033

    Enlarge

  27. pD649-HAsp-NRG1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156666
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NRG1 (NRG1 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156666-plasmid-map-sequence-id-302441

    Enlarge

  28. Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R]

    Plasmid
    #114507
    Purpose
    Mammalian Expression Plasmid of anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Human). Derived from hybridoma N120A/9.1.
    Depositor
    James Trimmer
    Article
    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1
    Insert
    anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Homo sapiens) recombinant mouse monoclonal antibody (NRG1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    dual CMV
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    114507-plasmid-map-sequence-id-245886

    Enlarge

  29. Neuregulin-CRD (Cysteine-rich domain, Type III) scFv [N126B/31]

    Plasmid
    #190528
    Purpose
    Mammalian Expression of Neuregulin-CRD (Cysteine-rich domain, Type III) scFV. Derived from hybridoma N126B/31.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Neuregulin-CRD (Cysteine-rich domain, Type III) (Homo sapiens) recombinant scFV (NRG1 Mouse)
    Use
    Tags
    HA, Sortase, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190528-plasmid-map-sequence-id-376403

    Enlarge

  30. Anti-Neuregulin-CRD [N126B/31-2b] - Chimeric

    Plasmid
    #227030
    Purpose
    Mammalian expression plasmid of anti-Neuregulin-CRD (Cysteine-rich domain, Type III) (Human). Derived from hybridoma N126B/31.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Neuregulin-CRD (Cysteine-rich domain, Type III) (Homo sapiens) recombinant mouse monoclonal antibody. (NRG1 Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227030-plasmid-map-sequence-id-451476

    Enlarge

Showing: 31 - 60 of 172 results