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pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
UseTagsChitin binding domain, His tag, and N-terminal Hi…ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK47
Plasmid#13324DepositorInsertgyrB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#154138PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEG03'
Plasmid#162578PurposeThis plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.DepositorInsertfucO, aldA
UseTagsExpressionBacterialMutationPromoterP_gyrAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
P48
Plasmid#212155PurposePlasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.DepositorInsertsuperfolder green fluorescent protein, secondary alcohol dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGU08
Plasmid#212149PurposePlasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.DepositorInsertcatalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDV02
Plasmid#212156PurposePlasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.DepositorInsertsecondary alcohol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGU06
Plasmid#212148PurposePlasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.DepositorInsertUseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mClover-YTH domain
Plasmid#177123PurposeBacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH domain) (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH domain only (IDR1 and IDR2 deletion)PromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTHDC1-FL
Plasmid#177121PurposeBacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mclover-YTH-IDR2
Plasmid#177122PurposeBacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
UseTagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH and IDR2 domains only (IDR1 deletion)PromoterT7Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_ccdB
Plasmid#204114PurposeType 0 CDS partDepositorInsertToxic DNA gyrase inhibitor ccdB
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB
Plasmid#131350PurposeExpresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase BDepositorInsertMyD88-GyrB (Myd88 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
N4177 strain
Bacterial Strain#13333DepositorBacterial ResistanceCoumermycinAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
JM109
Bacterial Strain#49761PurposeE.coli K-12 derivativeDepositorBacterial ResistanceNoneAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
JM107
Bacterial Strain#49759PurposeE.coli K-12 derivativeDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
JM106
Bacterial Strain#49757PurposeE.coli K-12 derivativeDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
DH5alpha::DddI
Bacterial Strain#179631PurposeFor expression of toxic DddADepositorBacterial ResistanceGentamicinAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
