-
Plasmid#226464PurposePromoterless MET predicted IRES between Rluc and FlucDepositorInsertPredicted MET IRES
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
RLuc_MET_95_FLuc
Plasmid#226463PurposeMET predicted IRES between Rluc and FlucDepositorInsertMET 5'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMetR
Plasmid#216329PurposeT7 promoter driving expression of metRDepositorInsertMetR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mAmetrine
Plasmid#228598PurposeMammalian expression of mAmetrine, for MuSIC barcode constructionDepositorInsertmAmetrine
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mAmetrine
Plasmid#228617PurposeMammalian expression of mAmetrine and extra insert, for MuSIC barcode constructionDepositorInsertmAmetrine
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Methylosarcina_fibrata
Plasmid#224406PurposeFor expression of the type I CBASS system encoded by Methylosarcina fibrataDepositorInsert4TM_MfCdnH_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ASPSCR1_METRNL
Plasmid#205775PurposeExpress mEGFP-tagged fusion protein, ASPSCR1_METRNL from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
Shim_MetRS_TP_GFP_pK7FWG2
Plasmid#202650PurposeDetermine where transit peptide targets GFP in N. benthamianaDepositorInsertN-terminal transit peptide from putative cytosolic MetRS
UseTagsGreen Florescent ProtienExpressionPlantMutationPromoterAvailable sinceAug. 10, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
eDrMet
Plasmid#183999PurposeOptically controlled eDrcMet expression in mammalian cellsDepositorInserteDrMet (MET Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_MET
Plasmid#183310PurposeAll-in-One CRISPRko system with a guide RNA that targets MET geneDepositorInsertMET
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMetLuc
Plasmid#135286PurposeGBPart - MetLuc Luciferase CDSDepositorInsertMetridia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ
Plasmid#172111PurposeExpression of Methionine Binding Protein (MetQ) with signal sequenceDepositorInsertMethionine Binding Protein
UseTags10x Histidine Tag and N.meningitidis signal seque…ExpressionBacterialMutationChanged Proline 22 to AlaninePromoterT7 PromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
NmMetNI-WT
Plasmid#172114PurposeExpression of MetN and MetI membrane proteinsDepositorInsertsMetN
MetI
UseTags10x His Tag and EnterokinaseExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_MET16
Plasmid#166091PurposePlasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertMET16 (MET16 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEtmem88aP1gfp
Plasmid#90132PurposeGateway Mid-entry clone containing promoter sequence specific for tmem88a driving expression of eGFPDepositorInserttmem88aP1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
PP-MetAP
Plasmid#40745PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-MetAP
UseTags6xHis TEVExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
Mouse lipid metabolism knockout library
Pooled Library#206248PurposeThe lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.DepositorUseCRISPR and LentiviralAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mouse sphingolipid metabolism knockout library
Pooled Library#206249PurposeThe sphingolipid metabolism library targets 82 mouse genes in the de novo ceramide and downstream sphingolipid metabolism pathways.DepositorUseCRISPR and LentiviralAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Lipid Droplet and Metabolism Library
Pooled Library#191535PurposeLentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPR Metabolic Gene Knockout Library
Pooled Library#110066PurposeEncodes gRNAs for targeting metabolic genes in CRISPR screens.DepositorUseLentiviralAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human Metabolic Gene CRISPRa sgRNA library
Pooled Library#187080PurposeLentiviral CRISPRa library targeting human metabolic genes for activation of gene expressionDepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mouse Metabolic Gene CRISPRa sgRNA library
Pooled Library#187081PurposeLentiviral CRISPRa library targeting mouse metabolic genes for activation of gene expressionDepositorExpressionMammalianSpeciesMus musculusUseLentiviralAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-metap2
Plasmid#100512PurposeExpresses HA tagged metap2 in mammalian cellsDepositorInsertMetap2 (METAP2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-METTL14-WT
Plasmid#141112PurposeOverexpression of WT METTL14 in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MetGFP
Plasmid#37560DepositorInsertMET (MET Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR-c-Met
Plasmid#31786DepositorInsertMet (MET Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
5TEY (METTL3)
Plasmid#101892PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMETTL3 (METTL3 Human)
UseBaculovirus expressionTags6xHis, TEVExpressionMutationPromoterpolyhedrinAvailable sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
5TEY (METTL14)
Plasmid#101891PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMETTL14 (METTL14 Human)
UseBaculovirus expressionTagsExpressionMutationPromoterpolyhedrinAvailable sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBad-mAmetrine1.1
Plasmid#18084PurposeBacterial expression of mAmetrine (version 1.1) fluorescent proteinDepositorInsertmAmetrine1.1
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBad-mAmetrine
Plasmid#18083PurposeBacterial expression of mAmetrine fluorescent proteinDepositorInsertmAmetrine fluorescent proteins
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET (V5 and HA)
Plasmid#222490PurposeCo-expression of two genes in yeast with V5 and HA tags and MET15 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37
Plasmid#196657PurposePlasmid for overexpression of native initiator tRNA (A37)DepositorInserttRNAfMet-A37 expression cassette
UseTagsExpressionBacterialMutationPromotermetY native promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet8
Plasmid#107449PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet8
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET8
Plasmid#107439PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET8 (MET8 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-U6-sgMettl3
Plasmid#196263PurposeExpresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoterDepositorInsertMettl3 sgRNA (Mettl3 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only