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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 151 results for: A1a

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Showing: 21 - 40 of 151 results

  3. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Article
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  4. SERPINA-bio-His

    Plasmid
    #52182
    Purpose
    Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SERPINA (SERPINA1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52182-plasmid-map-sequence-id-80945

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  11. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Construct Platinum TALEN vectors, with variable TALE repeats and non-RVD variations for expression in cultured cells, nematodes, sea urchins, ascidians, newts, frogs, mice, or rats.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Eukaryotic Expression, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Supplemental kit for #1000000024 includes modified pFUS vectors to simplify Golden Gate assembly and mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Reporters to measure receptor activation for G protein-coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. BCL2A1A

    Plasmid
    #42399
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BCL2A1A (BCL2A1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 151 results