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We narrowed to 10,970 results for: T7

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Showing: 21 - 40 of 10970 results

  1. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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  3. T7-VEE-GFP

    Plasmid
    #58977
    Purpose
    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Insert
    EGFP
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58977-plasmid-map-sequence-id-419580

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  13. pZB246-T7-T+

    Plasmid
    #250903
    Purpose
    Expresses N-terminal His-tag thermally stabilized T7 RNAP under a lac-inducible promoter on Kanamycin Resistance Backbone
    Depositor
    Timothy Whitehead
    Article
    Baumer et al bioRxiv [Preprint]. 2025 Nov 12:2025.11.
    Insert
    Thermally Stable T7 RNA Polymerase
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Arginine 31 to Aspartic Acid, Alanine 61 to Argin…
    Promoter
    pT5-lacUV
    Available Since
    Feb. 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    250903-plasmid-map-sequence-id-499452

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  19. T7-TetO-GFP_ColE1

    Plasmid
    #173193
    Purpose
    Expresses GFP under the control of a T7-TetO promoter. Expression can be repressed via TetR, and repression can be relieved via addition of tetracycline. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    T7-TetO
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    173193-plasmid-map-sequence-id-339027

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Showing: 21 - 40 of 10970 results