We narrowed to 7,473 results for: addgene/1000
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Plasmid#100037PurposeAAV expression of ArcLightD driven by hSynap promoterDepositorInsertArcLightD
UseAAVExpressionMammalianPromoterhSynapAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Frb-N-TEV
Plasmid#100095PurposeSNIPer plasmid, N terminal TEV fused to FrbDepositorInsertN-TEV
UseRetroviralTagsFrb and MycExpressionMammalianMutationN terminal domainPromoterCMVAvailable SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
HMBP-3C-STAR.66-285
Plasmid#100094Purposeexpresses MBP-tagged STARD1 protein domain (residues 66-285)DepositorAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-GFP
Plasmid#100009PurposeExpresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-GFPPromoterRibi promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
TagsV5 Epitope TagExpressionMammalianPromoterCMVAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-FOG1[N+C]
Plasmid#100085PurposedCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertFOG1(aa 1-45) (ZFPM1 Human)
UseCRISPRTags3XFLag-NLS-FOG1-dCas9-FOG1-NLSExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFX-Pickles 2.34NES
Plasmid#100028PurposeImproved FRET biosensor for the measurement of BCR-ABL activity.DepositorInsertPickles-2.34NES
UsePfx vector is derived from pcmv vector.ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40
Plasmid#100030PurposeAAV expression of ArcLightDDepositorInsertArcLightD
UseAAVTags2A-mCherryExpressionMammalianPromoterCBAAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-NavMs
Plasmid#100004PurposeElectrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFPDepositorInsertHis-NavMs, Voltage gated sodium channel
TagsHexa-His tag followed by a thrombin cleavage siteExpressionMammalianMutationC-terminus from a codon-optimized synthetic gene,…PromoterCMVAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn1.Twitch2B.WPRE.SV40
Plasmid#100040PurposeAAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorHas ServiceAAV1InsertTwitch2B
UseAAVExpressionMammalianPromoterhSyn1Available SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOD2047-bwmControl
Plasmid#100006PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
NavBp
Plasmid#100014PurposeElectrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFPDepositorInsertNavBp
ExpressionMammalianPromoterCMVAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
NsvBa
Plasmid#100015PurposeElectrophysiology in mammalian cells: Mammalian expression of a non-selective voltage gated ion channel and GFPDepositorInsertNsvBa
ExpressionMammalianPromoterCMVAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eGO-Matryoshka-LE-LP
Plasmid#100017PurposeBacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpEGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40
Plasmid#100038PurposeAAV expression of FLEXed ArcLightD driven by hSynap promoterDepositorInsertArcLightDco
UseAAVExpressionMammalianPromoterhSynapAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR
Plasmid#100008PurposeExpresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxPromoterRibi promoterAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only