We narrowed to 510 results for: piggybac vectors

1. PB_gRNA_S.pyogenes_scaffold_BB

   Plasmid

   #226429

   Purpose

   PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

   Depositor

   Alon Goren

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_p300_core-dCas9-EGFP_hygro

   Plasmid

   #226431

   Purpose

   PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

   Depositor

   Alon Goren

   Inserts

   p300 core-dCas9-EGFP fusion protein
   rtTA3-IRES-Hygro
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   HA tag, P2A-EGFP, and p300 core

   Expression

   Mammalian

   Mutation

   Promoter

   Dox inducible minimal CMV and UbC Promoter

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

   Plasmid

   #100597

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

   Plasmid

   #100596

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CA

   Plasmid

   #20960

   Purpose

   piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TET

   Plasmid

   #20909

   Purpose

   piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_Mut(D1399Y)_p300_core-dCas9-EGFP_hygro

   Plasmid

   #226427

   Purpose

   PiggyBac transposon vector containing a dox inducible mutant p300 core-dCas9 fusion protein (p300 core mutation: D1399Y) and EGFP reporter. Hygromycin selection marker.

   Depositor

   Alon Goren

   Inserts

   Mutant p300 core-dCas9-EGFP fusion protein
   rtTA3-IRES-Hygro
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   HA tag, Mutant p300 core, and P2A-EGFP

   Expression

   Mammalian

   Mutation

   p300 core mutation (D1399Y)

   Promoter

   Dox inducible minimal CMV and UbC Promoter

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TET-PH-lox66FRT

    Plasmid

    #139561

    Purpose

    piggyBac expression vector with TET-ON system and puromycin selection

    Depositor

    Minoru Ko

    Insert

    PGK-puroR-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TET-ZH-lox66FRT

    Plasmid

    #139563

    Purpose

    piggyBac expression vector with TET-ON system and Zeocin selection

    Depositor

    Minoru Ko

    Insert

    PGK-ZeoR-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-TET-BH-lox66FRT

    Plasmid

    #139562

    Purpose

    piggyBac expression vector with TET-ON system and Blasticidin selection

    Depositor

    Minoru Ko

    Insert

    PGK-BSD-pA cassette, inserted in PB-TET vector
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB Neo* PB

    Plasmid

    #138413

    Purpose

    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette

    Depositor

    Mario Capecchi

    Insert

    Neo*
    

    Use

    Mouse Targeting; Piggybac

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScarlessHD-DsRed

    Plasmid

    #64703

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    piggyBac-3xP3-DsRed
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    3xP3

    Available Since

    Dec. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-PB-R4R3

    Plasmid

    #139518

    Purpose

    Provides two PiggyBac transposon sequences between gene cassettes in the destination vector

    Depositor

    Minoru Ko

    Type

    Empty backbone

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TAC-ERP2

    Plasmid

    #80478

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TA-ERN

    Plasmid

    #80474

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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