We narrowed to 1,211 results for: vp64

1. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hUbC-VP64 dCas9 VP64-T2A-GFP

   Plasmid

   #59791

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized VP64 dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192665

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Cas9m4-VP64

   Plasmid

   #47319

   Purpose

   Cas9m4 Activator

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4-VP64
   

   Use

   CRISPR

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUBC_HA_2xPP7-VP64

   Plasmid

   #68418

   Purpose

   Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   PP7(deltaFG)
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   PP7: [delta]67_74 (non-aggregating)

   Promoter

   human UbC

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VP64-dCas9

   Plasmid

   #177171

   Purpose

   VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   VP64-dCas9(D10A, H840A)
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xFLag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF_dCas9-VP64

   Plasmid

   #68417

   Purpose

   Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and VP64

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUBC_HA_L7Ae-VP64

   Plasmid

   #68421

   Purpose

   Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   L7Ae
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Promoter

   human UbC

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAct:dCas9-VP64

    Plasmid

    #78901

    Purpose

    Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cas9m2-VP64

    Plasmid

    #47317

    Purpose

    Cas9m2 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m2-VP64
    

    Use

    CRISPR

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pAF1-VP64

    Plasmid

    #51427

    Purpose

    Artificial transcription factor with VP64

    Depositor

    Xavier Darzacq

    Article

    Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cas9m3-VP64

    Plasmid

    #47318

    Purpose

    Cas9m3 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m3-VP64
    

    Use

    CRISPR

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAct:MCP-VP64

    Plasmid

    #78904

    Purpose

    Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2:VP64
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    March 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Zdk3-VP64

    Plasmid

    #231571

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    Zdk3-VP64
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

    Plasmid

    #194279

    Purpose

    Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized VP64 dSaCas9 VP64 T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hU6 and hUbC

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid

    #66708

    Purpose

    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

    Depositor

    Charles Gersbach

    Insert

    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Cas9m4-VP64

    Plasmid

    #47319

    Purpose

    Cas9m4 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m4-VP64
    

    Use

    CRISPR

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUBC_HA_2xPP7-VP64

    Plasmid

    #68418

    Purpose

    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    PP7(deltaFG)
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    human UbC

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge