We narrowed to 2,224 results for: Pam

#160132

Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#160135

Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#160133

Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#195859

WT E. coli strain transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Serves as control for suppressor strains. No color when grown with or without rhamnose.

Gregory Phillips

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Feb. 8, 2023

Academic Institutions and Nonprofits only

#195858

E. coli nonsense suppressor strain (leuX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows magenta when CP is induced with rhamnose.

Gregory Phillips

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Feb. 8, 2023

Academic Institutions and Nonprofits only

#195857

E. coli nonsense suppressor strain (tyrT) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. No color when grown with or without rhamnose.

Gregory Phillips

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Feb. 8, 2023

Academic Institutions and Nonprofits only

#195856

E. coli nonsense suppressor strain (serU) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows salmon when CP is induced with rhamnose.

Gregory Phillips

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Feb. 8, 2023

Academic Institutions and Nonprofits only

#195855

E. coli nonsense suppressor strain (glnX) transformed with plasmid carrying chromogenic protein (CP) with amber nonsense mutation in chromophore. Grows purple when CP is induced with rhamnose.

Gregory Phillips

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Feb. 8, 2023

Academic Institutions and Nonprofits only