We narrowed to 6,333 results for: nls

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pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Plasmid

#176310

Purpose

Expresses CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

CreN59_nMag_NLS_P2A_mCherry_IRES_puroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

human EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45564

Purpose

Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

d14 eGFP donor
I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Mutation

deleted n' 15 amino acids

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pBLO1811_arC9_noNLS_human

Plasmid

#74494

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Use

CRISPR

Tags

t2a mCherry (C-terminal on insert)

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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p413_Csy4NLS

Plasmid

#103139

Purpose

Expresses Csy4-NLS under TEF promoter

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

Csy4

Tags

NLS

Expression

Yeast

Promoter

TEF

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

Plasmid

#45579

Purpose

Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani K227M

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pP2A_NLS_sfGFP

Plasmid

#63709

Purpose

Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm

Use

Just a donor dna with nanog homologous arm for kn…

Tags

sfGFP

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBLO1808_arC9_2xNLS_human

Plasmid

#74491

Purpose

Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

arC9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_SNAP_NR1

Plasmid

#170458

Purpose

Expresses SNAP tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSP1594_2x_NLS

Plasmid

#110625

Purpose

Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
None

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBLO1806_Cas9_2xNLS_human

Plasmid

#74493

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Tags

t2a mCherry

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

Plasmid

#220224

Purpose

AAV-STARR-seq validation control vector with Venus

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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NmeCas9_3XNLS_pMCSG7

Plasmid

#120078

Purpose

Bacterial expression of wtNmeCas9 with 3X NLS

Depositor

Erik Sontheimer

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

NmeCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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LI D-E C-NLS c2

Plasmid

#54428

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

NLS of SV40 large T antigen

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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GPKAnls

Plasmid

#61094

Purpose

GFP-tagged PKA catalytic subunit targeted to the nucleus by addition of a nuclear localization signal

Depositor

Steven Green

Article

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCAGEN_V5_eMagAF_C74_cMycNLS

Plasmid

#235640

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

V5-eMagAF and cMycNLS

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_RFP_WPRE_polyA

Plasmid

#220222

Purpose

AAV-STARR-seq validation vector with RFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

Plasmid

#220223

Purpose

AAV-STARR-seq validation control vector with TFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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