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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NINJ1

Plasmid

#201181

Purpose

Dox-inducible expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

TRE3G

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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PDZD7-TwinStrepII

Plasmid

#83198

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Article

Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

Insert

PDZD7 (Pdzd7 Mouse)

Use

Tags

Twin Strep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NINJ1

Plasmid

#201180

Purpose

Transient expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TetR-tdTomato

Plasmid

#212958

Purpose

piggyBac donor vector containing TetR-tdTomato for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetR-tdTomato

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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peCALWY-4

Plasmid

#22236

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

C418S mutation linker consists of 9 GGSGGS repea…

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

Plasmid

#164510

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Has Service

AAV9

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

Synapsin

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1209

Plasmid

#44491

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]

Use

Gateway expression clone

Tags

Expression

Mutation

Promoter

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
peCALWY-1

Plasmid

#22233

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-1 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

linker consists of 9 GGSGGS repeats

Promoter

Available since

Nov. 18, 2009

Availability

Academic Institutions and Nonprofits only
pET30a-FluA-NP

Plasmid

#165092

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJW31

Plasmid

#136423

Purpose

Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7

Depositor

Joseph Bondy-Denomy

Article

Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

Inserts

Cas5
Cas8
Cas7
Cas3
Cas3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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peCALWY-6

Plasmid

#22238

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY6 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

C416S mutation Identical to peCALWY-4, but linke…

Promoter

Available since

Jan. 5, 2010

Availability

Academic Institutions and Nonprofits only
peCALWY-5

Plasmid

#22237

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-5 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

C416S mutation identical to peCALWY-4, but linke…

Promoter

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
peCALWY-3

Plasmid

#22235

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-3 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

Similar to eCALWY-1, but with a linkerlength of 3…

Promoter

Available since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
peCALWY-2

Plasmid

#22234

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-2 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

sequence identical to eCALWY-1, but linkerlength …

Promoter

Available since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
pMLS791

Plasmid

#188889

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ1208

Plasmid

#44490

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]

Use

Gateway expression clone

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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