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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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ML1Nwt_in_pEGFP-C1

Plasmid

#67797

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-eIF4E1

Plasmid

#129644

Purpose

Express APEX2 fusion to cap-binding protein eIF4E1 in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

EIF4E1 (EIF4E Human)

Tags

APEX2

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pCDNA3

Plasmid

#107174

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hFGD5

Plasmid

#50760

Purpose

expression of EGFP-tagged human FGD5

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

FGD5 (FGD5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-TRE3G-PYL1-sfGFP-PML

Plasmid

#167011

Purpose

expression of PYL1-SfGFP-PML, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

PML (PML Human)

Use

Lentiviral

Tags

GFP-PML

Expression

Mammalian

Available Since

June 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-TSG101

Plasmid

#116925

Purpose

expresses mEGFP-TSG101 in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

TSG101 (TSG101 Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

Plasmid

#169449

Purpose

expression of TRF1-mcherrry and sgRNA targeting telomeres

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

mCherry-TRF1

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo_pAS32

Plasmid

#118222

Purpose

expresses 3xHA-tagged miniTurbo in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6M-PAK1 T423E

Plasmid

#12208

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Activated Pak1: T423E

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-TurboID-NES

Plasmid

#116904

Purpose

Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 K299R

Plasmid

#12210

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive Pak1: K299R

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

Plasmid

#179585

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NLS-NG-T2A

Promoter

CAGp

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pCS2+8-CYP2A6-flag-tev-his

Plasmid

#227165

Purpose

For overexpression of CYP2A6 in mammalian cells and purification.

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag and His6

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Article

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6M-PAK1 H83L H86L K299R

Plasmid

#26592

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

H83L H86L K299R Dominant negative

Available Since

March 9, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-LifeAct-FRB-Venus-173-N

Plasmid

#87616

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-Venus173-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHis APEX2-eIF4A1

Plasmid

#129645

Purpose

Express APEX2 fusion to DEAD-box translation factor eIF4A1 in bacteria

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

eukaryotic translation initiation factor 4A1 (EIF4A1 Human)

Tags

6xHis and APEX2

Expression

Bacterial

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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