We narrowed to 790 results for: p65

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pGEX-4T Syt1 C2AB(140) F234C

Plasmid

#134697

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

F234C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) V304C

Plasmid

#134698

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

V304C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T Syt1 C2AB(140) I367C

Plasmid

#134699

Purpose

Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

Depositor

Edwin Chapman

Article

Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

Insert

Synaptotagmin 1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

I367C, C277A

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B V304A,I367A IRES GFP

Plasmid

#133821

Purpose

Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

V304A, I367A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A K189-192A IRES GFP

Plasmid

#133823

Purpose

Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K189-192A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A M173,F234A IRES GFP

Plasmid

#133825

Purpose

Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

M173, F234A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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PB-UniSAM

Plasmid

#99866

Purpose

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

Depositor

Lesley Forrester

Article

Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

Insert

UniSAM-mCherry + U6-gRNA2.0

Use

CRISPR and Synthetic Biology; Dcas9-sam activation

Expression

Mammalian

Mutation

BbsI sites in CDS were ablated by consensus mutat…

Promoter

EF1a

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEBB HA RelA

Plasmid

#74892

Purpose

Express RelA in mammalian cells

Depositor

Ezra Burstein, Colin Duckett

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

RelA (RELA Human)

Tags

HA

Expression

Mammalian

Promoter

EF-1alpha promoter

Available Since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pYpet-PBD

Plasmid

#22781

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pBabe-Ypet-PBD

Plasmid

#24533

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Use

Retroviral

Tags

Ypet

Expression

Mammalian

Available Since

Aug. 19, 2010

Availability

Academic Institutions and Nonprofits only
pCMV6M-Pak1 H83L H86L

Plasmid

#12211

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

PBD (p21-binding domain) mutant: H83L, H86L (see …

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pET23-PBD(65-150)-N-Cys

Plasmid

#13722

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD(65-150)-N-Cys (PAK1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Met-Cys-Gly-PBD(65-150)-

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pCXLE-dCas9VPPH-T2A-GFP-shP53

Plasmid

#102897

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9VPPH-T2A-GFP-shP53

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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AA199

Plasmid

#216050

Purpose

Fragmid fragment: (C' terminus) p65; NpNLS

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

p65_v1.1; NpNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 (96-421)

Plasmid

#213619

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 5W

Plasmid

#213623

Purpose

Bacterial expression of SUMO-tagged rat syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 s7+

Plasmid

#213624

Purpose

Bacterial expression of SUMO-tagged rat syt1 s7+

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 s7+ (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgRELA guide 1

Plasmid

#193591

Purpose

RELA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRELA guide 1 (RELA Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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