We narrowed to 4,719 results for: pcr

1. pCri_L706-antiSH2-OptoMB

   Plasmid

   #159935

   Purpose

   Opto-MB bacterial expression

   Depositor

   Jared Toettcher, Jose Avalos

   Article

   Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

   Insert

   anti-SH2 MB with SS58 insert
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRT7 NT Topo arabinose

   Plasmid

   #73446

   Purpose

   Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides

   Depositor

   Jesse Rinehart

   Type

   Empty backbone

   Use

   Tags

   GST (N-terminal), 6xHis (C-terminal)

   Expression

   Bacterial

   Mutation

   Promoter

   pBad

   Available Since

   March 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8/GW/TOPO-ERF

   Plasmid

   #98626

   Purpose

   Gateway entry TOPO TA cloning vector ERF

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ERF (Erf Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR8/GW/TOPO-ELK4

   Plasmid

   #98617

   Purpose

   Gateway entry TOPO TA cloning vector for ELK4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK4 (Elk4 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

   Plasmid

   #89887

   Purpose

   Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-HRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

   Plasmid

   #89886

   Purpose

   Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-NRasQ61K
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC149-pCR8-dCas9VP160

   Plasmid

   #48221

   Purpose

   dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP160

   Expression

   Mutation

   D10A;H840A nuclease-deficient

   Promoter

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR-Flag-IKKalpha-KM

   Plasmid

   #15468

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase alpha-KM (Chuk Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Lysine at 44 to Alanine

   Promoter

   Available Since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pCRISPaint-NanoLuc-PuroR

   Plasmid

   #80962

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   NanoLuc
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC1816_pCR8-gSMN2-DN4

    Plasmid

    #118649

    Purpose

    Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS505 EF1alpha-EGFPcre*

    Plasmid

    #11955

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pCRISPaint-ProteoTuner DD-PuroR

    Plasmid

    #80964

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    ProteoTuner tag
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCruzHA SIRT1 G261A

    Plasmid

    #10963

    Depositor

    Toren Finkel

    Article

    Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G261A

    Promoter

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pBS500 EF1alpha-GFPcre

    Plasmid

    #11920

    Depositor

    Brian Sauer

    Article

    Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

    Insert

    GFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pCR-Flag-IKKbeta-KM

    Plasmid

    #15466

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase beta-KM (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Lysine at 44 to Alanine

    Promoter

    Available Since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pAC84-pCR8-dCas9

    Plasmid

    #48218

    Purpose

    dCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A)
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag

    Expression

    Mutation

    D10A;H840A

    Promoter

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC1823_pCR8-RBFOX1N-dCasRx-C

    Plasmid

    #118657

    Purpose

    Gateway Donor; RBFOX1N-dCasRx-C ORF; No promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-dCasRx-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR8/GW/TOPO-ETV1

    Plasmid

    #98623

    Purpose

    Gateway entry TOPO TA cloning vector for ETV1

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ETV1 (Etv1 Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR3.1 GSK3beta S9A HA

    Plasmid

    #15994

    Depositor

    Morris Birnbaum

    Article

    Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-

    Insert

    GSK3beta (GSK3B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S9A: constitutively active

    Promoter

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pAC1813_pCR8-gSMN2-DN1

    Plasmid

    #118646

    Purpose

    Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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