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We narrowed to 514 results for: sp2

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pISH-Gap43

Plasmid

#74336

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

growth associated protein 43 (Gap43 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMP416

Plasmid

#134996

Purpose

dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

Depositor

Ahmad Khalil

Article

Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

Insert

pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)

Use

Synthetic Biology

Tags

3xFLAG, E coli Dam(N132A), and dCas9

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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HA, EGFP Spastin M87 with exon 4

Plasmid

#167542

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Use

Tags

EGFP and HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 S245X

Plasmid

#92366

Purpose

expression of M1 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_NEBL-1/1

Plasmid

#91383

Purpose

Protein expression and purification of human SH3 domain construct NEBL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEBL-1/1 (NEBL Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3B/WT myc-M87

Plasmid

#87722

Purpose

Expresses WT myc-M87 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M87 isoform (deletion of nt 1-490 - 5'UTR an…

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 S245X

Plasmid

#92368

Purpose

expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP N184X with 5'UTR

Plasmid

#92375

Purpose

Inducible expression of N184X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

S184X

Promoter

Tet-responsive P tight

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-SPARTIN, residues 1-101

Plasmid

#180626

Purpose

Bacterial expression for SPARTIN MIT residues 1-101. Has N-terminal HIS-GST tag. Internal ID: WISP20-57.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPARTIN

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-101

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-CALPAIN-7 1-165

Plasmid

#180610

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165. Has N-terminal cleavable HIS-GST tag.Internal ID: WISP21-129

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-65

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165

Plasmid

#180611

Purpose

Bacterial expression vector for CALPAIN-7 residues 1-165, tandem MIT domains. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-25

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Rtn2b-R60f-mCherry

Plasmid

#186607

Purpose

Third generation lentiviral vector expressing Reticulon 2 disease mutant R60f fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 mutant R60f (RTN2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

c.178insC (R60f)

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-NTD136-271-mNeon

Plasmid

#186604

Purpose

Encodes human Reticulon 2 isoform B N-terminal domain aa 136 to 271 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 136 to 271 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 N184X

Plasmid

#92373

Purpose

Inducible expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

Tet-responsive P tight

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP S245X with 5'UTR

Plasmid

#92376

Purpose

Inducible expression of S245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

S245X

Promoter

Tet-responsive P tight

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 S245X

Plasmid

#92374

Purpose

Inducible expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

Tet-responsive P tight

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 N184X

Plasmid

#92371

Purpose

Inducible expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

Tet-responsive P tight

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/SP S245X with 5'UTR

Plasmid

#92370

Purpose

expression of 245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

S245X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 N184X

Plasmid

#92367

Purpose

expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge