164,728 results

1. pETMCSI-T4P_Y320A

   Plasmid

   #122522

   Purpose

   Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

   Depositor

   Gottfried Otting

   Article

   Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

   Insert

   gp43 - DNA polymerase (43 Escherichia virus T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   tyrosine 320 changed to alanine

   Promoter

   T7 promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_TRET_hNgn2_UBC_Puro

   Plasmid

   #61474

   Purpose

   3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

   Depositor

   Ron Weiss

   Article

   Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

   Inserts

   Ngn2 (NEUROG2 Human)
   Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

   Plasmid

   #179106

   Purpose

   pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAP-AAV.FUS.3

   Plasmid

   #222333

   Purpose

   AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

   Depositor

   Mikhail Shapiro, Jerzy Szablowski

   Article

   Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

   Insert

   AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p41

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-Con/Fon-GCaMP6M (AAV8)

   Viral Prep

   #137119-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-EF1a-Con/Fon-GCaMP6M (#137119). In addition to the viral particles, you will also receive purified pAAV-EF1a-Con/Fon-GCaMP6M plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6M. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   None

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

6. p45sheLL

   Plasmid

   #37323

   Depositor

   John Schiller

   Article

   Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

   Insert

   HPV45L1+L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRKH3-ermGFP

   Plasmid

   #27169

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   presence of an additional 183-bp region at N term…

   Promoter

   Available since

   Feb. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIN4-CMV-K2M

   Plasmid

   #21164

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Klf4, cMyc (MYC, KLF4 Human)
   

   Use

   Lentiviral; Reprogramming

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1A-CD19.8H.8TM.BB.3z

   Plasmid

   #200671

   Purpose

   Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHN924

    Plasmid

    #178885

    Purpose

    Expression of GST-His8-HRV3C

    Depositor

    Kazuhiro Abe

    Insert

    human rhinovirus 3C protease
    

    Use

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit

    #1000000191

    Purpose

    Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

    Depositor

    Arnold Driessen

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

12. pGP-CMV-GCaMP6s

    Plasmid

    #40753

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6s
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

    Plasmid

    #83841

    Purpose

    Fluorescent probe for G1/S transition and M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-LYCipi

    Plasmid

    #53279

    Purpose

    Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    idi
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human CRISPR Metabolic Gene Knockout Library

    Pooled Library

    #110066

    Purpose

    Encodes gRNAs for targeting metabolic genes in CRISPR screens.

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Use

    Lentiviral

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

16. mNeonGreen-mTurquoise2

    Plasmid

    #98886

    Purpose

    Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC64420

    Plasmid

    #209517

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC7A5

    Plasmid

    #132197

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A5 (SLC7A5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Inducible Caspex expression

    Plasmid

    #97421

    Purpose

    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

    Depositor

    Steven Carr, Samuel Myers

    Article

    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

    Inserts

    Caspex
    T2A-Gfp
    

    Use

    CRISPR

    Tags

    3xFLAG and V5

    Expression

    Mammalian

    Mutation

    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

    Promoter

    TRE3G

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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