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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

Plasmid

#211969

Purpose

Express gRNA against LIN28A with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting LIN28A (LIN28A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

Plasmid

#211968

Purpose

Express gRNA against LIN28A with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting LIN28A (LIN28A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

Plasmid

#207355

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#182078

Purpose

Mammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-SPHKAP [L131/17R]

Plasmid

#177477

Purpose

Mammalian Expression Plasmid of anti-SPHKAP (Mouse). Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SPHKAP (Mus musculus) recombinant mouse monoclonal antibody (Sphkap Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

Plasmid

#211966

Purpose

Express gRNA against JARID2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting JARID2 (JARID2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(G12V)-IRES-p53DD

Plasmid

#233185

Purpose

Retroviral expression of HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/27]

Plasmid

#190502

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-10xHis

Plasmid

#194178

Purpose

Expresses MPP8-10xHis tagged

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Kir6.1 K+ channel scFv [N366/60]

Plasmid

#190547

Purpose

Mammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpM6 [N470/22R]

Plasmid

#206596

Purpose

Mammalian Expression Plasmid of anti-TrpM6 (Human). Derived from hybridoma N470/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM6 (Homo sapiens) recombinant Mouse monoclonal antibody (TRPM6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav1.2 Ca2+ channel scFv [N263/31]

Plasmid

#182083

Purpose

Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-SHLD3

Plasmid

#114123

Purpose

Expresses N-terminally tagged FLAG-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-FLAG-SHLD1

Plasmid

#114115

Purpose

Expresses N-terminally tagged FLAG-SHLD1 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD1 (SHLD1 Human)

Use

Genomic integration, tetracycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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Kir2.1 K+ channel scFv [N112/16]

Plasmid

#206769

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112/16 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Kir2.1 K+ channel scFv [N112B/29]

Plasmid

#206770

Purpose

Mammalian Expression of Kir2.1 K+ channel scFV. Derived from hybridoma N112B/29 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir2.1 K+ channel (Mus musculus) recombinant scFV (Kcnj2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-OCRL/INPP5b [N166A/26]

Plasmid

#190301

Purpose

Mammalian Expression Plasmid of anti-OCRL/INPP5b (Human). Derived from hybridoma N166A/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-OCRL/INPP5b (Homo sapiens) recombinant Mouse monoclonal antibody (OCRL Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-6 [N182/17R-2b]

Plasmid

#206661

Purpose

Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-6 (Homo sapiens) recombinant Mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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