We narrowed to 15,102 results for: gnal

1. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. RapR-Shp2-mVenus-flag

   Plasmid

   #188655

   Purpose

   Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   Shp2
   

   Tags

   Flag and mVenus

   Expression

   Mammalian

   Mutation

   iFKBP insertion at V406 with deletion of residues…

   Promoter

   CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-Arl13b2

   Plasmid

   #40871

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Use

   Gateway entry

   Mutation

   no stop codon

   Available Since

   Nov. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

4. FKBP_cPPVp

   Plasmid

   #118967

   Purpose

   Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cPPVp in fusion with FKBP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB_nPPVp

   Plasmid

   #118966

   Purpose

   Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nPPVp in fusion with FRB
   

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFlox_N-RPB1_BSD_FKBPF36V_EYFP

   Plasmid

   #163884

   Purpose

   Acute Depletion of RBP1-EYFP

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Rbp1 (Polr2a Mouse)
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mVenus-RnBarr2

   Plasmid

   #137799

   Purpose

   Visualization of beta-arrestin2

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Rat beta-arrestin2 (Arrb2 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLIC-myc-GBAi

   Plasmid

   #171753

   Purpose

   For the mammalian expression of myc-GBAi

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCS2 Flag Smad3 EPSM V179T

   Plasmid

   #26998

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Xenopus/mammalian/avian/zebrafish

   Tags

   Flag

   Mutation

   T179V S204A S208A S213A; V179T

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pECFP-Rac1Y40C

    Plasmid

    #105293

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSVE Grb2 myc 49L

    Plasmid

    #26085

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 P49L (GRB2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    P49L

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

13. PhyB-mCherry-NLS

    Plasmid

    #51571

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    NLS and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. DEP-GFP

    Plasmid

    #109043

    Purpose

    Expression of the Dishevelled DEP domain

    Depositor

    Mariann Bienz

    Article

    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

    Insert

    dishevelled segment polarity protein 2 (DVL2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Dvl2 DEP domain

    Promoter

    CMV

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIN-Ski-gRNA2

    Plasmid

    #180369

    Purpose

    targeting mouse Ski gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Ski targeting gRNA (Ski Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-MCPIP1(delta ZF)

    Plasmid

    #90110

    Purpose

    Mammalian expression of Flag-MCPIP1(delta ZF)

    Depositor

    Mingui Fu

    Article

    Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

    Insert

    MCPIP1 (ZC3H12A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K317G, S318G

    Promoter

    CMV

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH-SFB-RNF169

    Plasmid

    #99392

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

    Depositor

    Michael Huen

    Article

    Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

    Insert

    RNF169 (RNF169 Human)
    

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW002-Pc-TorA(sp)

    Plasmid

    #111253

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA signal peptide
    

    Expression

    Bacterial

    Promoter

    Pc

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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