We narrowed to 4,107 results for: lenti crispr plasmid

1. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Plasmid

   #154430

   Purpose

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

   Depositor

   David Liu

   Article

   Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

   Insert

   hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   VRQR point mutations in SpCas9

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLeGO.sgGata1.4.RUNX1A.iG2

   Plasmid

   #181976

   Purpose

   gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

   Depositor

   Jan-Henning Klusmann

   Inserts

   GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
   RUNX1A (RUNX1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLD-puro-Cc-CR-PARK7WT-VA

   Plasmid

   #115187

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD-puro-Cc-CR-PARK7V51G-VA

   Plasmid

   #115188

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-Cc-CR-PARK7C53A-VA

   Plasmid

   #115189

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLD-puro-Cc-CR-PARK7H126A-VA

   Plasmid

   #115190

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLD-puro-Cc-CR-PARK7E163K-VA

   Plasmid

   #115191

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   CR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Mutation

   c.150G>A (silent when translated to protein, c…

   Promoter

   CMV

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107311

   Purpose

   Encoding scFv-p65-HSF1 driven by EF1α promoter

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2_mClover3_LacZ

    Plasmid

    #155103

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LCV2_mCherry_LacZ_sgRNA

    Plasmid

    #155108

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LCV2_mCherry_Luciferase_sgRNA

    Plasmid

    #155109

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    Luciferase_sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV.U6gT28.13.EFS-NS.H2B-RFP

    Plasmid

    #170388

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Promoter

    EFS-NS

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV.U6gT28.4.EFS-NS.H2B-RFP

    Plasmid

    #170365

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Promoter

    EFS-NS

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.U6gT28.3.EFS-NS.H2B-RFP

    Plasmid

    #170364

    Purpose

    Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Promoter

    EFS-NS

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCHKOv3-mClover-intergenic_2-As

    Plasmid

    #218814

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCHKOv3-mClover-intergenic_3-As

    Plasmid

    #218815

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKOv3-mClover-intergenic_4-As

    Plasmid

    #218816

    Purpose

    Lentiviral vector expressing mClover3 along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR_PGK_antiCD19_eZ3_Gal4VP64

    Plasmid

    #169917

    Purpose

    Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

    Depositor

    Zhili Rong

    Article

    Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

    Insert

    antiCD19-Notch3-GL4VP64
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Additional EGF repeat between the extracellular d…

    Promoter

    PGK

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(hBIRC2_1k)-PGKpuro2ABFP-W

    Plasmid

    #208416

    Purpose

    Lentiviral gRNA plasmid targeting human BIRC2 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

    Insert

    BIRC2 (BIRC2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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