We narrowed to 17,417 results for: She

1. pFRT-TODestGFP_HuB

   Plasmid

   #65761

   Purpose

   expresses GFP-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5 FRT-Hygro hTERT FL-Firefly

   Plasmid

   #171057

   Purpose

   hTERT Full-length-expressing splicing minigene

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   hTERT FL-Firefly
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTXB1-CII

   Plasmid

   #51566

   Purpose

   Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

   Depositor

   Francisco Malagon, Sankar Adhya

   Insert

   cII
   

   Use

   Tags

   Intein-Chiting Binding Domanin

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSADdeltaG-GFP-mCherry

   Plasmid

   #32643

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Inserts

   GFP
   mCherry
   

   Use

   Rabies virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pMT-HA-STX1

   Plasmid

   #89310

   Purpose

   Expresses HA tagged syntaxin 1 in Drosophila cells

   Depositor

   Andrew Peden

   Insert

   STX1 (Syx1A Fly)
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   Promoter

   P-MT (Metallothionein)

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBbS1c-RFP

   Plasmid

   #35338

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MSCV--Mouse Mdm2 wt-IRES-CFP

   Plasmid

   #180493

   Purpose

   Role of Mdm2 splicing variants in tumorigenesis

   Depositor

   Martine Roussel

   Article

   Dang et al Cancer Res. 2002 Feb 15;62(4):1222-30.

   Insert

   Mdm2 full length (wt)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBACgus4x/cdc37/LRRK2 1867-2186

   Plasmid

   #40364

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   HisTev

   Expression

   Mutation

   WT aa1867-2186

   Promoter

   polH

   Available since

   Oct. 2, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pRN3P_TIR1

   Plasmid

   #119766

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   TIR1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFRT-TODestFLAGHA_HuD

    Plasmid

    #65757

    Purpose

    expresses FLAGHA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNIC28-hKdm2b-CxxC-PHD

    Plasmid

    #49216

    Purpose

    Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

    Depositor

    Rob Klose

    Insert

    Kdm2b (KDM2B Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Hs.NRAS Q61L

    Plasmid

    #83178

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61L

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. COVID-SARS2 NSP16/10

    Plasmid

    #159615

    Purpose

    Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

    Depositor

    Opher Gileadi

    Inserts

    Covid-SARS2 Nsp16
    Covid-SARS2 Nsp10
    

    Use

    Tags

    His6-Zb-TEV and none

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    (Bicistronic) and T7 - LacO

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Expression

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRN3P_Kdm5b_MUT

    Plasmid

    #119767

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    Kdm5b MUT
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFRT-DestFLAGHA_HuD

    Plasmid

    #65760

    Purpose

    constitutively expresses FLAG-HA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pBbE6c-RFP

    Plasmid

    #35287

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBbS2k-RFP

    Plasmid

    #35330

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBbE8c-RFP

    Plasmid

    #35269

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDest eGFP CUGBP1

    Plasmid

    #61276

    Purpose

    Mammalian expression of EGFP-tagged human CUGBP1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CUG-BP (CELF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 4H4H

    Plasmid

    #51314

    Depositor

    Cheryl Arrowsmith

    Insert

    4H4H (EHMT1 4H4H, Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pLEX307-APN-G418

    Plasmid

    #158456

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Hs.HRAS Q61R

    Plasmid

    #83188

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61R

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid

    #78336

    Purpose

    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    PAX3 (PAX3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pDONR221-FURIN

    Plasmid

    #158442

    Purpose

    Gateway Cloning compatible entry vector for the human FURIN gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    FURIN (FURIN Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSA101

    Plasmid

    #59787

    Purpose

    drives ZIF-1 protein in C. elegans neurons

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    rab-3 promoter
    zif-1 coding (zif-1 Nematode)
    unc-54 3' UTR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMT-TAK1-V5-His

    Plasmid

    #63753

    Purpose

    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    TGF-β activated kinase 1 (Tak1 Fly)
    

    Use

    Tags

    V5-His

    Expression

    Insect

    Mutation

    Promoter

    pMT

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUG-amdSYM-mak10TR

    Plasmid

    #75433

    Purpose

    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

    Depositor

    John McCusker

    Inserts

    MAK10TR
    ApR
    amdSYM
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminal 740 bp of MAK10 ORF

    Promoter

    Native promoter and PTEF

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Hs.HRAS G12D

    Plasmid

    #83183

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    G12D

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBbB5a-GFP

    Plasmid

    #35352

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. NEK2

    Plasmid

    #39163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NEK2 (NEK2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    T175A inactivating mutant

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

32. MARS

    Plasmid

    #205232

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    

    Use

    Tags

    Nuclear Export Sequence and mScarlet-i

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR221-CTSB

    Plasmid

    #158443

    Purpose

    Gateway Cloning compatible entry vector for the human CTSB gene.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    CTSB (CTSB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBbA8k-RFP

    Plasmid

    #35273

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Hs.NRAS Q61K

    Plasmid

    #83180

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Q61K

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNC-BiFC-ED1

    Plasmid

    #193426

    Purpose

    Nimble cloning-compatible vector for plant BiFC

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. K560GFP_17NL

    Plasmid

    #129767

    Purpose

    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    DAL insert before A345

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-cytoMetGFP

    Plasmid

    #37561

    Depositor

    David Rimm

    Insert

    MET (MET Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    only contains cytoplasmic domain amino acids K956…

    Promoter

    CMV

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TAF1

    Plasmid

    #39118

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1 (TAF1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

40. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid

    #78347

    Purpose

    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only