We narrowed to 4,749 results for: NAP

1. rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

   Plasmid

   #32481

   Purpose

   Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAML141F,Y115F-GlyR
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   Synapsin

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

   Plasmid

   #74513

   Purpose

   AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

   Depositor

   Guoping Feng

   Article

   Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

   Insert

   EGFP-p2A-EGFP-f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBRα-σ70 D581G

   Plasmid

   #53732

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The sigma 70 moiety carries the D581G substitution

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pJM189A

   Plasmid

   #218135

   Purpose

   For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to ZF degron SD0.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD0
   

   Use

   Tags

   rpoZ

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSW GAP43-jRGECO1a P2A Syp-Halo

   Plasmid

   #185565

   Purpose

   Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

   Insert

   GAP43-jRGECO1a P2A Syp-Halo
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-syn-fRGECO2

   Plasmid

   #125244

   Purpose

   Expresses red fluorescent calcium sensor in hippocampal slices

   Depositor

   Katalin Torok

   Article

   Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

   Insert

   fRGECO2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W44Y, D432A

   Promoter

   synapsin

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NK73

   Plasmid

   #176609

   Purpose

   To test bead engulfment of macrophages.

   Depositor

   Meghan Morrissey

   Article

   Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

   Insert

   SNAP-CD86-FCERG (CD86 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human RNF149-3X MYC

   Plasmid

   #225333

   Purpose

   Lentiviral expression of human RNF149 with 3x myc tag in mammalian cells

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   RNF149 (RNF149 Human)
   

   Use

   Lentiviral

   Tags

   3xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLP-pTac-Tn5-chlorR

   Plasmid

   #199994

   Purpose

   Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Landing Pads
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

    Plasmid

    #89570

    Purpose

    Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Inserts

    Channelrhodopsin
    Histone 2B
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

    Plasmid

    #113760

    Purpose

    AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

    Depositor

    Luke Lavis

    Article

    Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

    Insert

    tdNfsB(F124W)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F124W

    Promoter

    Synapsin

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-synP-FLEX-splitTVA-EGFP

    Plasmid

    #52474

    Purpose

    Expresses splitTVA-EGFP

    Depositor

    Ian Wickersham

    Article

    Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

    Insert

    splitTVA-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin-1

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM-AAV-mSyn1-EGFP

    Plasmid

    #153187

    Purpose

    Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcPylRS(Y384F Y306A)

    Plasmid

    #196486

    Purpose

    To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP

    Plasmid

    #32479

    Purpose

    Cre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAML141F-GlyR-IRES-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    Synapsin

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

    Plasmid

    #136916

    Purpose

    Expresses ChR2(H134R)-EYFP in Cre-negative cells

    Depositor

    Ofer Yizhar

    Article

    Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H134R in ChR2

    Promoter

    Human Synapsin1

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

    Plasmid

    #114212

    Purpose

    Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

    Depositor

    Xue Han

    Article

    Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    human synapsin (hSyn)

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. hSyn-GW-dCas9-SET2-IRES2-mCherry

    Plasmid

    #202089

    Purpose

    Neuron-specific expression vector of dCas9-Set2

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM33_PEX14_Pex14

    Plasmid

    #178484

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PEX14_Pex14 (PEX14 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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