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pAAV-EF1a-C-CreintG

Plasmid

#69571

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Has service

AAV1

Insert

kozak consensus - C-CreintG

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NEDD4 (NEDD4 Human)

Tags

HA

Expression

Mammalian

Mutation

C867A

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-EF1a-FLEX-TVA-mCherry

Plasmid

#38044

Depositor

Naoshige Uchida

Publication

Watabe-Uchida et al Neuron. 2012 Jun 7;74(5):858-73.

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)

Use

AAV and Cre/Lox

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32481

Purpose

Cre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Publication

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

AAV and Cre/Lox; Adeno-associated virus, flex swi…

Tags

IRES-EGFP

Promoter

Synapsin

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCF CREB M1

Plasmid

#22969

Depositor

Marc Montminy

Publication

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CREB (Creb1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Available since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FCK-Mac-GFP

Plasmid

#22223

Purpose

Lentiviral expression of Mac-GFP for optogenetic inhibition

Depositor

Edward Boyden

Publication

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

NO

Promoter

CaMKII

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3 S6K2 WT

Plasmid

#17729

Depositor

John Blenis

Publication

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

S6K2 (RPS6KB2 Human)

Tags

HA

Expression

Mammalian

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Publication

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Publication

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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1051 pBabe puroL Akt

Plasmid

#9011

Depositor

William Sellers

Publication

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
MSCV puro let-7 sponge

Plasmid

#29766

Depositor

Phil Sharp

Publication

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

let-7 sponge

Use

Retroviral

Expression

Mammalian

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
SARS-CoV2 Nucleocapsid

Plasmid

#153201

Purpose

Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

Depositor

Peter Klein

Publication

Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))

Tags

Myc tag (5X)

Expression

Mammalian

Promoter

CMV

Available since

July 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

5 Articles

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CFP-C1-PLCdelta-PH

Plasmid

#21262

Depositor

Tobias Meyer

Publication

Botelho et al J Cell Biol. 2000 Dec 25. 151(7):1353-68

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLCdelta (PLCD1 Human)

Tags

CFP

Expression

Mammalian

Mutation

PH domain of PLCdelta (human). Also known as PLCd…

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-Neo-Bam p53 V143A

Plasmid

#16435

Depositor

Bert Vogelstein

Publication

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p53 V143A (TP53 Human)

Expression

Mammalian

Mutation

V143A. Mutant cDNA from human colorectal tumor CX…

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Publication

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-PBD-EYFP

Plasmid

#13723

Depositor

Klaus Hahn

Publication

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PBD-EYFP (PAK1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

p21 binding domain of human Pak1(amino acids 65-1…

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSF107mT-GATEWAY-3'-FLAG

Plasmid

#67619

Purpose

A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Publication

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Flag

Expression

Mammalian

Promoter

CMV and SP6

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-MKL2

Plasmid

#27175

Purpose

For mammalian expression of human MKL2 with a 3x FLAG tag

Depositor

Ron Prywes

Publication

Selvaraj et al J Biol Chem. 2003 Oct 24. 278(43):41977-

Insert

MKL2 (MRTFB Human)

Tags

3XFLAG

Expression

Mammalian

Available since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles